Wong N L, Magil A B, Dirks J H
Department of Medicine, University of British Columbia, Vancouver, Canada.
Nephron. 1989;51(1):84-8. doi: 10.1159/000185248.
Clearance experiments were conducted with metabolic cages to determine the effect of dietary magnesium on gentamicin nephrotoxicity. Three groups of male Wistar rats were given low, medium- or high-magnesium diets. Following baseline clearances, gentamicin was administered by intramuscular injection (20 mg/kg). Additional clearances were performed 6 and 11 days after gentamicin administration. 24-hour clearances were also taken 8 days after gentamicin withdrawal. The present experiments demonstrated that high magnesium intake protected the kidney against injury induced by gentamicin. This reduction in nephrotoxicity was probably due to competition of binding between magnesium and gentamicin to the renal membrane. Histological examinations were also done in these animals. The results showed that the most severe changes were seen in rats receiving a low-magnesium diet. Rats given a high-magnesium diet showed the least toxic changes while rats receiving a medium-magnesium diet showed changes of intermediate severity. These observations complemented the results obtained from 24-hour clearances and indicate the protective effect of dietary magnesium on the development of acute renal failure following administration of gentamicin.
使用代谢笼进行清除实验，以确定膳食镁对庆大霉素肾毒性的影响。将三组雄性Wistar大鼠分别给予低镁、中镁或高镁饮食。在测定基线清除率后，通过肌肉注射给予庆大霉素（20mg/kg）。在给予庆大霉素后第6天和第11天进行额外的清除率测定。在停用庆大霉素8天后也进行24小时清除率测定。本实验表明，高镁摄入可保护肾脏免受庆大霉素诱导的损伤。肾毒性的降低可能是由于镁与庆大霉素在肾膜上的结合竞争所致。还对这些动物进行了组织学检查。结果显示，在接受低镁饮食的大鼠中观察到最严重的变化。给予高镁饮食的大鼠毒性变化最小，而接受中镁饮食的大鼠显示出中等程度的变化。这些观察结果补充了从24小时清除率获得的结果，并表明膳食镁对庆大霉素给药后急性肾衰竭的发展具有保护作用。
