Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., Kanagawa, Japan.
Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., Kanagawa, Japan.
Regul Toxicol Pharmacol. 2018 Feb;92:94-103. doi: 10.1016/j.yrtph.2017.11.009. Epub 2017 Nov 21.
The recent rapid increase in the prevalence of emerging tobacco- and nicotine-containing products, such as e-cigarettes, is being driven in part by their reduced-risk potential compared to tobacco smoking. In this study, we examined emission levels for selected cigarette smoke constituents, so-called "Hoffmann analytes", and in vitro toxicity of aerosol from a novel tobacco vapor product (NTV). The NTV thermally vaporizes a nicotine-free carrier liquid to form an aerosol which then passes through tobacco, where it absorbs tobacco-derived flavors and nicotine. The NTV results were compared with those for 3R4F cigarette smoke. Chemical analysis of the NTV aerosol demonstrated that Hoffmann analyte levels were substantially lower than in 3R4F smoke and that the most were below quantifiable levels. Results from in vitro bacterial reverse mutation, micronucleus and neutral red uptake assays showed that, in contrast with 3R4F smoke, the NTV aerosol failed to demonstrate any measurable genotoxicity or cytotoxicity. The temperature of tobacco during NTV use was measured at approximately 30 °C, which may explain the lower Hoffmann analyte emission and in vitro toxicity levels. These results suggest that the aerosol from the NTV has a very different toxicological profile when compared with combustible cigarette smoke.
最近，电子烟等新型烟草和含尼古丁产品的流行率迅速上升，部分原因是它们与吸烟相比潜在风险降低。在这项研究中，我们检测了一种新型烟草蒸气产品（NTV）产生的气溶胶中选定的香烟烟雾成分（所谓的“霍夫曼分析物”）的排放水平和体外毒性。NTV 通过热蒸发不含尼古丁的载体液体形成气溶胶，然后通过烟草，在烟草中吸收烟草衍生的风味和尼古丁。将 NTV 的结果与 3R4F 香烟烟雾的结果进行了比较。对 NTV 气溶胶的化学分析表明，霍夫曼分析物的水平明显低于 3R4F 烟雾，其中大部分低于可量化水平。细菌回复突变、微核和中性红摄取测定的体外结果表明，与 3R4F 烟雾相反，NTV 气溶胶未能表现出任何可测量的遗传毒性或细胞毒性。NTV 使用过程中烟草的温度约为 30°C，这可能解释了霍夫曼分析物排放和体外毒性水平较低的原因。这些结果表明，与可燃香烟烟雾相比，NTV 产生的气溶胶具有非常不同的毒理学特征。
