Mae Shin-Ichi, Ryosaka Makoto, Toyoda Taro, Matsuse Kyoko, Oshima Yoichi, Tsujimoto Hiraku, Okumura Shiori, Shibasaki Aya, Osafune Kenji
Center for iPS Cell Research and Application (CiRA), Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Center for iPS Cell Research and Application (CiRA), Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Biochem Biophys Res Commun. 2018 Jan 1;495(1):954-961. doi: 10.1016/j.bbrc.2017.11.105. Epub 2017 Nov 20.
Recent progress in kidney regeneration research is noteworthy. However, the selective and robust differentiation of the ureteric bud (UB), an embryonic renal progenitor, from human pluripotent stem cells (hPSCs) remains to be established. The present study aimed to establish a robust induction method for branching UB tissue from hPSCs towards the creation of renal disease models. Here, we found that anterior intermediate mesoderm (IM) differentiates from anterior primitive streak, which allowed us to successfully develop an efficient two-dimensional differentiation method of hPSCs into Wolffian duct (WD) cells. We also established a simplified procedure to generate three-dimensional WD epithelial structures that can form branching UB tissues. This system may contribute to hPSC-based regenerative therapies and disease models for intractable disorders arising in the kidney and lower urinary tract.
肾脏再生研究的最新进展值得关注。然而，从人多能干细胞（hPSCs）中选择性且强有力地分化出输尿管芽（UB）（一种胚胎肾祖细胞）的方法仍有待确立。本研究旨在建立一种从hPSCs中强有力地诱导出分支状UB组织的方法，以用于创建肾脏疾病模型。在此，我们发现前侧中间中胚层（IM）从前侧原条分化而来，这使我们成功开发出一种将hPSCs高效二维分化为中肾管（WD）细胞的方法。我们还建立了一个简化程序来生成可形成分支状UB组织的三维WD上皮结构。该系统可能有助于基于hPSC的再生疗法以及用于肾脏和下尿路难治性疾病的疾病模型。
