Mae Shin-Ichi, Ryosaka Makoto, Osafune Kenji
Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
Methods Mol Biol. 2019;1926:117-123. doi: 10.1007/978-1-4939-9021-4_10.
The generation of ureteric bud (UB), which is the renal progenitor that gives rise to renal collecting ducts and lower urinary tract, from human-induced pluripotent stem cells (hiPSCs) provides a cell source for studying the development of UB and kidney disease. Here we describe a stepwise and efficient two-dimensional differentiation method of hiPSCs into Wolffian duct (WD) cells. We also describe how to generate three-dimensional WD epithelial structures that can differentiate into UB-like structures.
输尿管芽（UB）是一种肾祖细胞，可分化为肾集合管和下尿路，从人诱导多能干细胞（hiPSC）中生成输尿管芽为研究输尿管芽发育和肾脏疾病提供了细胞来源。在此，我们描述了一种将hiPSC逐步高效地二维分化为中肾管（WD）细胞的方法。我们还描述了如何生成可分化为输尿管芽样结构的三维中肾管上皮结构。
