Dias-Souza Marcus Vinícius, Soares Daniel Lucas, Dos Santos Vera Lúcia
Microbiology Department, Biological Sciences Institute, Federal University of Minas Gerais, MG, Brazil.
Faculty of Pharmacy, Federal University of Minas Gerais, MG, Brazil.
Saudi Pharm J. 2017 Nov;25(7):999-1004. doi: 10.1016/j.jsps.2017.03.003. Epub 2017 Mar 12.
This study aimed to investigate for the first time the antibiofilm effectiveness of two chloramphenicol liposome formulations against biofilms of potentially pathogenic bacteria associated to corrosion isolated from the water of cooling towers from a Brazilian industry. Antibiofilm assays with liposomes were performed in 96-wells microtiter plates, and data was compared to free chloramphenicol treatment. Chloramphenicol-loaded liposomes were successfully produced using the dehydration-rehydration method, with vesicle diameters of 131 nm (100 nm membrane extrusion) and 182 nm (200 nm membrane extrusion) assessed by dynamic light scattering. The liposomes obtained by 100 nm membrane extrusion were more effective than 200 nm membrane extrusion vesicles against the biofilms after overnight exposure, and the free drug had no antibiofilm effect. Our study open doors for more investigations on liposome entrapment of antimicrobial compounds such as biocides of industrial use, for controlling biofilm formation in aquatic environments.
本研究旨在首次调查两种氯霉素脂质体制剂对从巴西一家工业冷却塔水中分离出的与腐蚀相关的潜在致病细菌生物膜的抗生物膜效果。在96孔微量滴定板中进行了脂质体的抗生物膜试验，并将数据与游离氯霉素处理进行比较。采用脱水再水化法成功制备了载氯霉素脂质体，通过动态光散射评估，囊泡直径分别为131nm（100nm膜挤压）和182nm（200nm膜挤压）。过夜暴露后，通过100nm膜挤压获得的脂质体对生物膜的效果比200nm膜挤压囊泡更有效，而游离药物没有抗生物膜作用。我们的研究为进一步研究脂质体包裹抗菌化合物（如工业用杀菌剂）以控制水生环境中生物膜的形成打开了大门。
