Samanfar Bahram, Shostak Kristina, Moteshareie Houman, Hajikarimlou Maryam, Shaikho Sarah, Omidi Katayoun, Hooshyar Mohsen, Burnside Daniel, Márquez Imelda Galván, Kazmirchuk Tom, Naing Thet, Ludovico Paula, York-Lyon Anna, Szereszewski Kama, Leung Cindy, Jin Jennifer Yixin, Megarbane Rami, Smith Myron L, Babu Mohan, Holcik Martin, Golshani Ashkan
Department of Biology and Ottawa Institute of Systems Biology, Carleton University, Ottawa, Ontario, Canada.
Agriculture and Ari-Food Canada, Ottawa Research and Development Centre (ORDC), Ottawa, Ontario, Canada.
PeerJ. 2017 Nov 14;5:e4037. doi: 10.7717/peerj.4037. eCollection 2017.
The presence of acetic acid during industrial alcohol fermentation reduces the yield of fermentation by imposing additional stress on the yeast cells. The biology of cellular responses to stress has been a subject of vigorous investigations. Although much has been learned, details of some of these responses remain poorly understood. Members of heat shock chaperone HSP proteins have been linked to acetic acid and heat shock stress responses in yeast. Both acetic acid and heat shock have been identified to trigger different cellular responses including reduction of global protein synthesis and induction of programmed cell death. Yeast and code for two important heat shock proteins that together account for 1-2% of total cellular proteins. Both proteins have been linked to responses to acetic acid and heat shock. In contrast to the overall rate of protein synthesis which is reduced, the expression of and is induced in response to acetic acid stress. In the current study we identified two yeast genes and that are linked to acetic acid and heat shock sensitivity. We investigated the influence of these genes on the expression of HSP proteins. Our observations suggest that and influence translation in a CAP-independent manner.
工业酒精发酵过程中乙酸的存在会给酵母细胞带来额外压力，从而降低发酵产量。细胞对应激反应的生物学机制一直是大量研究的主题。尽管已经了解了很多，但其中一些反应的细节仍知之甚少。热休克伴侣HSP蛋白家族成员已被证明与酵母中的乙酸和热休克应激反应有关。乙酸和热休克都能引发不同的细胞反应，包括整体蛋白质合成的减少和程序性细胞死亡的诱导。酵母 和 编码两种重要的热休克蛋白，它们总共占细胞总蛋白的1-2%。这两种蛋白都与乙酸和热休克反应有关。与整体蛋白质合成速率降低相反， 和 的表达在乙酸应激反应中被诱导。在本研究中，我们鉴定了两个与乙酸和热休克敏感性相关的酵母基因 和 。我们研究了这些基因对HSP蛋白表达的影响。我们的观察结果表明， 和 以不依赖CAP的方式影响翻译。
