Zhao Dong-Hao, Ke Chang-Liang, Liu Qi, Wang Xu-Feng, Wang Qiang, Li Liu-Dong
Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, Guangzhou, 510300, People's Republic of China.
Laboratiry of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation, Guangzhou, 510300, People's Republic of China.
BMC Vet Res. 2017 Nov 21;13(1):346. doi: 10.1186/s12917-017-1273-3.
Fish are vulnerable to stress from over-crowding during transportation and eugenol is the most common sedative used to minimize fish injury. The ADI value of 2.5 mg/kg is recommended by the Joint FAO/WHO Expert Committee on Food Additives. The aim of this work was to study the elimination kinetics of eugenol following exposure of grass carp to a eugenol bath in a simulated transportation setting.
Grass carp, Ctenopharyngodon idella (120 fish) were exposed for 24 h to a 10 mg/L eugenol bath. Sampling was performed during a 96 h period after the 24 h bath. Eight fish were sampled at each time point and muscle, plasma and liver concentrations of the drug were determined by ultra-performance liquid chromatography-tandem mass spectrometry. The concentration-time data of eugenol in each tissue were analyzed using non-compartmental methods. The peak concentrations (C) in plasma, muscle and liver were 7.68, 5.30 and 24.63 mg/kg and the elimination half-lives (t) were 19.79, 10.27 and 55.28 h, respectively. The clearance (CL) values were 0.10, 0.44 and 0.04 L/h/kg and the areas under the concentration-time curve (AUC) were 91.54, 22.44, and 214.12 mg·h/L in plasma, muscle and liver, respectively. After a eugenol exposure bath, drug concentrations in muscle tissue of grass carp were below 1 mg/kg at 8 h and 0.1 mg/kg at 24 h.
The drug concentrations in muscle tissue at 8 h were lower than the recommended ADI value.
鱼类在运输过程中容易受到过度拥挤的压力，丁香酚是最常用的镇静剂，用于尽量减少鱼类损伤。联合国粮食及农业组织/世界卫生组织食品添加剂联合专家委员会建议的每日允许摄入量（ADI）值为2.5毫克/千克。这项工作的目的是研究在模拟运输环境中，草鱼暴露于丁香酚浴后丁香酚的消除动力学。
120条草鱼（Ctenopharyngodon idella）暴露于10毫克/升的丁香酚浴中24小时。在24小时浴后的96小时内进行采样。每个时间点采集8条鱼，通过超高效液相色谱-串联质谱法测定药物在肌肉、血浆和肝脏中的浓度。使用非房室模型方法分析丁香酚在每个组织中的浓度-时间数据。血浆、肌肉和肝脏中的峰值浓度（C）分别为7.68、5.30和24.63毫克/千克，消除半衰期（t）分别为19.79、10.27和55.28小时。清除率（CL）值分别为0.10, 0.44和0.04升/小时/千克，血浆、肌肉和肝脏中浓度-时间曲线下面积（AUC）分别为91.54、22.44和214.12毫克·小时/升。经过丁香酚暴露浴后，草鱼肌肉组织中的药物浓度在8小时时低于1毫克/千克，在24小时时低于0.1毫克/千克。
8小时时肌肉组织中的药物浓度低于推荐的ADI值。
