Xu Yidan, Jiao Yaqin, Yang Jian, Tan Aijuan, Ou Deyuan, Song Xuqin, Lv Shiming
Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, Guizhou, China.
College of Life Science, Guizhou University, Guiyang, Guizhou, China.
Front Vet Sci. 2023 Jan 25;9:1097812. doi: 10.3389/fvets.2022.1097812. eCollection 2022.
The pharmacokinetic profile and residue depletion of eugenol in carp () tissues and plasma were performed by a convenient and reliable high-performance liquid chromatography (HPLC) method.
The eugenol in carp tissues and plasma was extracted with a mixed solution of acetonitrile and methanol. N-hexane was used to remove lipid impurities. The method was successfully applied to the pharmacokinetic and residue elimination of eugenol in carp after the carp was administered a medicated bath.
The average recoveries of eugenol in tissues and plasma fortified with four concentration levels were 69.0-106.6% and 80.0-86.7%, respectively. The relative standard deviations were < 8.9%. The limit of detection (LOD) was 0.01 μg/g in tissue and 0.008 μg/ml in plasma, respectively. The pharmacokinetic parameter of C for eugenol in plasma at the concentrations of 20, 35, and 75 mg/L were 10.86, 17.21, and 37.32 mg/L, respectively. The t values were 3.68, 4.22, and 9.31 h. After the investigation of the anesthetic effect, 35 mg/L of eugenol was the optimal concentration for anesthesia. The highest accumulation concentration of eugenol in carp is in the liver and the lowest is in the muscle. In addition, the eugenol in tissue was eliminated rapidly and at a lower level than the LOD at 48 h. According to the residue elimination, the withdrawal time of eugenol was suggested at 5.2 days.
These results indicate that the developed method had good linearity and accuracy, and is sensitive enough for the monitoring of eugenol residue in carp. The half-life of eugenol decreased with the increase in drug concentration and the eugenol was eliminated rapidly in carp tissues. 35 mg/L eugenol was recommended as an anesthetic in carp due to its favorable anesthetic effect and no mortality. This study will contribute to the establishment of MRL regulation and setting a withdrawal period.
采用简便可靠的高效液相色谱（HPLC）法研究了丁香酚在鲤鱼组织和血浆中的药代动力学特征及残留消除情况。
用乙腈和甲醇混合溶液提取鲤鱼组织和血浆中的丁香酚。用正己烷去除脂质杂质。该方法成功应用于鲤鱼药浴后丁香酚的药代动力学和残留消除研究。
四个浓度水平加标的组织和血浆中丁香酚的平均回收率分别为69.0% - 106.6%和80.0% - 86.7%。相对标准偏差<8.9%。组织中的检测限（LOD）为0.01μg/g，血浆中的检测限为0.008μg/ml。丁香酚在血浆中浓度为20、35和75mg/L时的药代动力学参数C分别为10.86、17.21和37.32mg/L。t值分别为3.68、4.22和9.31小时。在研究麻醉效果后，35mg/L的丁香酚是最佳麻醉浓度。鲤鱼中丁香酚的最高蓄积浓度在肝脏，最低在肌肉。此外，组织中的丁香酚在48小时时迅速消除且低于检测限水平。根据残留消除情况，建议丁香酚的休药期为5.2天。
这些结果表明所建立的方法具有良好的线性和准确性，对监测鲤鱼中丁香酚残留足够灵敏。丁香酚的半衰期随药物浓度增加而降低，且在鲤鱼组织中迅速消除。由于其良好的麻醉效果且无死亡率，推荐35mg/L丁香酚作为鲤鱼麻醉剂。本研究将有助于建立最大残留限量规定和设定休药期。
