Portieles Roxana, Ochagavia María Elena, Canales Eduardo, Silva Yussuan, Chacón Osmani, Hernández Ingrid, López Yunior, Rodríguez Mayra, Terauchi Ryohei, Borroto Carlos, Santos Ramón, Bolton Melvin D, Ayra-Pardo Camilo, Borrás-Hidalgo Orlando
Center for Genetic Engineering and Biotechnology, 10600, Havana, Cuba.
Tobacco Research Institute, Carretera de Tumbadero 8, 6063, San Antonio de los Baños, Havana, Cuba.
BMC Res Notes. 2017 Nov 21;10(1):603. doi: 10.1186/s13104-017-2934-9.
The ubiquitous soil pathogen Rhizoctonia solani causes serious diseases in different plant species. Despite the importance of this disease, little is known regarding the molecular basis of susceptibility. SuperSAGE technology and next-generation sequencing were used to generate transcript libraries during the compatible Nicotiana tabacum-R. solani interaction. Also, we used the post-transcriptional silencing to evaluate the function of a group of important genes.
A total of 8960 and 8221 unique Tag sequences identified as differentially up- and down-regulated were obtained. Based on gene ontology classification, several annotated UniTags corresponded to defense response, metabolism and signal transduction. Analysis of the N. tabacum transcriptome during infection identified regulatory genes implicated in a number of hormone pathways. Silencing of an mRNA induced by salicylic acid reduced the susceptibility of N. tabacum to R. solani. We provide evidence that the salicylic acid pathway was involved in disease development. This is important for further development of disease management strategies caused by this pathogen.
普遍存在的土壤病原菌立枯丝核菌会在不同植物物种中引发严重病害。尽管这种病害很重要，但对于易感性的分子基础却知之甚少。利用超级SAGE技术和新一代测序技术，在烟草与立枯丝核菌的亲和互作过程中构建转录文库。此外，我们运用转录后沉默技术来评估一组重要基因的功能。
共获得8960个和8221个分别被鉴定为差异上调和下调的独特标签序列。基于基因本体分类，一些注释的单标签对应于防御反应、代谢和信号转导。对感染期间烟草转录组的分析确定了涉及多种激素途径的调控基因。水杨酸诱导的一种mRNA的沉默降低了烟草对立枯丝核菌的易感性。我们提供的证据表明水杨酸途径参与了病害发展。这对于进一步制定由该病原菌引起的病害管理策略很重要。
