Department of Medicine and Health Sciences "V. Tiberio", University of Molise, Campobasso, Italy.
Oxid Med Cell Longev. 2017;2017:2825703. doi: 10.1155/2017/2825703. Epub 2017 Oct 15.
Citicoline and homotaurine are renowned compounds that exhibit potent neuroprotective activities through distinct molecular mechanisms. The present study was undertaken to demonstrate whether cotreatment with citicoline and homotaurine affects cell survival in primary retinal cultures under experimental conditions simulating retinal neurodegeneration. Primary cultures were obtained from the retina of fetal rats and exposed to citicoline plus homotaurine (100 M). Subsequently, neurotoxicity was induced using excitotoxic levels of glutamate and high glucose concentrations. The effects on retinal cultures were assessed by cell viability and immunodetection of apoptotic oligonucleosomes. The results showed that a combination of citicoline and homotaurine synergistically decreases proapoptotic effects associated with glutamate- and high glucose-treated retinal cultures. This study provides an insight into the potential application of citicoline and homotaurine as a valuable tool to exert neuroprotective effects against retinal damage.
胞磷胆碱和牛磺酸是两种著名的化合物，它们通过不同的分子机制发挥强大的神经保护作用。本研究旨在证明在模拟视网膜神经退行性变的实验条件下，胞磷胆碱和牛磺酸联合治疗是否会影响原代视网膜培养物中的细胞存活。原代培养物取自胎鼠视网膜，并暴露于胞磷胆碱加牛磺酸（100 μM）中。随后，用兴奋性谷氨酸和高葡萄糖浓度诱导神经毒性。通过细胞活力和凋亡寡核苷酸体的免疫检测评估对视网膜培养物的影响。结果表明，胞磷胆碱和牛磺酸联合使用可协同降低与谷氨酸和高葡萄糖处理的视网膜培养物相关的促凋亡作用。这项研究为胞磷胆碱和牛磺酸作为一种有价值的工具，发挥对视网膜损伤的神经保护作用提供了新的见解。
