Ivanishko Yury, Bravin Alberto, Kovalev Sergey, Lisutina Polina, Lotoshnikov Mikhail, Mittone Alberto, Tkachev Sergey, Tkacheva Marina
Rostov Eye Clinic "InterYUNA", Rostov-on-Don, Russia.
Rostov State Medical University, Central Scientific Research Laboratory, Rostov-on-Don, Russia.
Invest Ophthalmol Vis Sci. 2017 Nov 1;58(13):5941-5948. doi: 10.1167/iovs.17-22273.
The intracranial three-dimensional (3D) visualization of the whole volume of the eyeball at micrometric resolution has not been achieved yet either in clinical nor in preclinical diagnostic research. Overcoming this limitation may provide a new tool for clinical and preclinical studies of different pathologies of the various sections of the eye. The aim of this work is to give the first insight of a volumetric visualization at the high resolution of the entire enucleated and intracranial postmortem rabbit eyeballs.
X-ray computed tomography phase-contrast imaging was used to obtain 3D models of enucleated and intracranial rabbit eyes. Images were compared with the ones measured by using optical coherence tomography (OCT) images. The experiment was carried out at the European Synchrotron Radiation Facility.
Combining the unique possibilities offered by phase-contrast imaging, microtomography, and the properties of synchrotron radiation, the 3D visualization of the whole eyeball, at an isotropic voxel size of 3.1 μm3, is reported here for the first time.
High image contrast is achieved without the necessity of injection of contrast agents, thanks to the superior performances, achieved by x-ray phase-contrast imaging with respect to the conventional radiographic imaging. The measurement protocol developed within this work opens the way for in vivo high-resolution visualization of the entire organ.
无论是在临床诊断研究还是临床前诊断研究中，尚未实现以微米级分辨率对整个眼球体积进行颅内三维（3D）可视化。克服这一限制可能为眼部不同部位各种病变的临床和临床前研究提供一种新工具。这项工作的目的是首次展示整个摘除眼球并置于颅内的死后兔眼球的高分辨率体积可视化情况。
使用X射线计算机断层扫描相衬成像来获取摘除眼球并置于颅内的兔眼的3D模型。将图像与使用光学相干断层扫描（OCT）图像测量的图像进行比较。实验在欧洲同步辐射装置进行。
结合相衬成像、显微断层扫描的独特可能性以及同步辐射的特性，本文首次报道了在各向同性体素大小为3.1μm³的情况下对整个眼球进行3D可视化。
由于X射线相衬成像相对于传统放射成像具有卓越性能，无需注射造影剂即可实现高图像对比度。本研究中开发的测量方案为整个器官的体内高分辨率可视化开辟了道路。
