European Synchrotron Radiation Facility, ID17 Grenoble, France.
Rostov Eye Clinic "InterYUNA", Rostov-on-Don, Russia; Rostov State Medical University, Central Scientific Research Laboratory, Rostov-on-Don, Russia.
Phys Med. 2018 Jul;51:7-12. doi: 10.1016/j.ejmp.2018.06.004. Epub 2018 Jun 15.
Several complementary methods able to visualize the internal structures of eyes are used in the clinical practice in the diagnosis of pathologies affecting a specific zone of the eye. Despite the significant technological progress, the visualization of the entire eyeball at micrometric resolution is yet an unsolved task both in clinical diagnostics and in laboratory research. With this respect, high resolution 3D images of the eyeball would be extremely useful, in the study of various pathologies of the retina, the lens, and of the optic nerve. In this work we combined the state-of-the-art of micro computed tomography technology with phase-contrast imaging, a recent highly sensitive technique well adapted to investigate soft tissues without the use of contrast agents; we applied the technique in the post-mortem analysis of monkey eyes, which share several similitudes with the human organ. We reported here vascular, nervous and anatomical details of monkey eyes imaged with a 3.1 × 3.1 × 3.1 µm voxel size as well as the first 3D visualisation of the entire globe of Macaca's fascicularis eye. Results have also been compared with, and validated by, histological analysis.
在临床实践中，有几种互补的方法可用于诊断影响眼睛特定区域的病变，这些方法能够观察眼睛的内部结构。尽管技术取得了重大进展，但在临床诊断和实验室研究中，仍未解决以亚毫米分辨率可视化整个眼球的问题。在研究视网膜、晶状体和视神经的各种病变时，高分辨率的眼球 3D 图像将非常有用。在这项工作中，我们将微计算机断层扫描技术的最新技术与相衬成像相结合，这是一种最近的高灵敏度技术，非常适合在不使用造影剂的情况下研究软组织；我们将该技术应用于猴子眼睛的死后分析，猴子的眼睛与人眼有许多相似之处。我们在这里报告了用 3.1μm×3.1μm×3.1μm 体素大小成像的猴子眼睛的血管、神经和解剖细节，以及 Macaca fascicularis 眼睛整个球体的首次 3D 可视化。结果还与组织学分析进行了比较和验证。
