Adebayo O A, Akinloye O, Adaramoye O A
Faculty of Basic Medical Sciences, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Faculty of Basic Medical Sciences, Department of Medical Laboratory Science, University of Lagos, Lagos, Nigeria.
Andrologia. 2018 Apr;50(3). doi: 10.1111/and.12920. Epub 2017 Nov 21.
The toxicity of metallic nanoparticles is a growing concern due to its application in industries and homes. We investigated the toxicity of cerium oxide nanoparticles (CeO NPs) on reproductive system in male balb/c mice. Twenty mice were divided into four groups of five animals each and treated thus: normal saline (control), 100, 200 and 300 μg/kg CeO NPs (i.p.,) thrice in a week for five consecutive weeks. Results showed that CeO NPs significantly reduced the levels of haemoglobin, PCV and RBC count relative to controls. In addition, luteinising and follicle-stimulating hormones (FSH and LH) and prolactin were significantly reduced in the mice. Specifically, CeO NPs at 100 μg/kg decreased testosterone by 23%, while CeO NPs at 200 μg/kg decreased FSH, LH and prolactin by 25%, 26% and 13%, respectively. Testicular malondialdehyde was increased by 103%, 106% and 135% in mice treated with 100, 200 and 300 μg/kg CeO NPs, respectively. CeO NPs caused a significant reduction in activities of antioxidant enzymes and levels of reduced glutathione and total nitric oxide. Moreso, CeO NPs decreased sperm motility and count and increased total sperm abnormality in mice. Histology revealed congestion and degeneration of seminiferous tubules. Overall, CeO NPs induces testicular dysfunction via disruption of antioxidant/oxidant balance and endocrine suppression.
由于金属纳米颗粒在工业和家庭中的应用，其毒性问题日益受到关注。我们研究了氧化铈纳米颗粒（CeO NPs）对雄性Balb/c小鼠生殖系统的毒性。将20只小鼠分为四组，每组五只，并进行如下处理：生理盐水（对照组），100、200和300μg/kg CeO NPs（腹腔注射），每周三次，连续五周。结果显示，相对于对照组，CeO NPs显著降低了血红蛋白、红细胞压积和红细胞计数水平。此外，小鼠体内的黄体生成素、促卵泡激素（FSH和LH）以及催乳素水平均显著降低。具体而言，100μg/kg的CeO NPs使睾酮水平降低了23%，而200μg/kg的CeO NPs分别使FSH、LH和催乳素水平降低了25%、26%和13%。在分别用100、200和300μg/kg CeO NPs处理的小鼠中，睾丸丙二醛含量分别增加了103%、106%和135%。CeO NPs导致抗氧化酶活性、还原型谷胱甘肽水平和总一氧化氮水平显著降低。此外，CeO NPs降低了小鼠的精子活力和数量，并增加了精子的总异常率。组织学检查显示生精小管充血和变性。总体而言，CeO NPs通过破坏抗氧化/氧化平衡和抑制内分泌诱导睾丸功能障碍。
