Teo Peishan, Wang Xiaowen, Zhang Jieying, Zhang Han, Yang Xin, Huang Yun, Tang Jintian
a Key Laboratory of Particle & Radiation Imaging of Ministry of Education, Department of Engineering Physics , Tsinghua University , Beijing , P.R. China.
b Yuquan Hospital, Medical Center , Tsinghua University , Beijing , P.R. China.
J Biomater Sci Polym Ed. 2018 Feb;29(2):181-194. doi: 10.1080/09205063.2017.1409048. Epub 2017 Nov 28.
To find a promising drug carrier to suppress tumor using magnetic induction hyperthermia (MIH) and targeted therapy, two superparamagnetic iron oxide nanoparticles (SPIONs) coated with polyethylene glycol (PEG) and LyP-1, respectively, were prepared and compared. The particle size ranges of PEG-SPIONs and LyP-1-SPIONs were 10-15 nm, and 15-20 nm, respectively. In FTIR spectra, PEG-SPIONs and LyP-1-SPIONs had strong peaks between 575 and 1630 cm. Specifically, the PEG-SPIONs mainly has peaks in 581 and 1630 cm. The LyP-1-SPIONs mainly had peaks in 575, 1050 and 1625 cm. The contents of FeO in the PEG-SPIONs and LyP-1-SPIONs were about 94.24 and 89.26%, respectively. The iron contents in the MCF-7 and CT-26 cells were 33.1 ± 1.8 and 27.9 ± 0.95 pg, respectively, after co-incubation with LyP-1-SPIONs for 8 h. The LyP-1-SPIONs accumulated in the nucleus of MCF-7 cells while PEG-SPIONs in cytoplasma. In vitro, after 30 days we can found the tumor almost stopped to grow in Group LyP-1-SPIONs. LyP-1-SPIONs are promising in treating cancer as they accumulated in the nucleus of MCF-7 cells which expressed p32 and almost stopped tumor growth by combined MIH and targeted therapy.
为了找到一种利用磁感应热疗（MIH）和靶向治疗来抑制肿瘤的有前景的药物载体，分别制备并比较了两种分别包覆聚乙二醇（PEG）和LyP-1的超顺磁性氧化铁纳米颗粒（SPIONs）。PEG-SPIONs和LyP-1-SPIONs的粒径范围分别为10 - 15纳米和15 - 20纳米。在傅里叶变换红外光谱（FTIR）中，PEG-SPIONs和LyP-1-SPIONs在575至1630厘米之间有强峰。具体而言，PEG-SPIONs主要在581和1630厘米处有峰。LyP-1-SPIONs主要在575、1050和1625厘米处有峰。PEG-SPIONs和LyP-1-SPIONs中FeO的含量分别约为94.24%和89.26%。与LyP-1-SPIONs共孵育8小时后，MCF-7和CT-26细胞中的铁含量分别为33.1±1.8和27.9±0.95皮克。LyP-1-SPIONs积聚在MCF-7细胞的细胞核中，而PEG-SPIONs积聚在细胞质中。在体外，30天后我们发现LyP-1-SPIONs组的肿瘤几乎停止生长。LyP-1-SPIONs在治疗癌症方面具有前景，因为它们积聚在表达p32的MCF-7细胞的细胞核中，并通过联合MIH和靶向治疗几乎阻止了肿瘤生长。
