Department of Radiology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine (TCM), Nanjing 210029 Jiangsu, China.
Department of Radiology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine (TCM), Nanjing 210029 Jiangsu, China.
Mater Sci Eng C Mater Biol Appl. 2014 Aug 1;41:161-7. doi: 10.1016/j.msec.2014.04.041. Epub 2014 Apr 28.
Polyethylene glycol (PEG)-coated superparamagnetic iron oxide nanoparticles (PEG·SPIONs) were prepared by a facile one-pot approach. The synthesized PEG·SPIONs were found to be uniform in size with an average hydrodynamic diameter of 11.7 nm. PEG·SPIONs exhibited excellent dispersibility in water, colloidal stability, and biocompatibility. The magnetic resonance imaging (MRI) properties of PEG·SPIONs were characterized both in vitro and in vivo. The dual contrast both in T1 and T2-weighted imaging was well enhanced with longitudinal and transverse relaxivity (r1, r2) of 35.92 s(-1) per mM of Fe(3+) and 206.91 s(-1) per mM of Fe(3+) respectively. In vivo T2-weighted MRI shows pronounced enhancement in the liver and spleen but not in T1-weighted MRI. Accumulations of nanoparticles were found primarily in the liver, spleen, and intestine, while much lower uptake in the kidney, heart, and lungs. A gradual excretion of PEG·SPIONs was observed via hepatobiliary (HB) processing over a period of 14 days. The toxicity of PEG·SPIONs was also evaluated in vitro and in vivo. PEG·SPIONs were found to be biocompatible by investigating organ tissues after hematoxylin-eosin staining. The conclusion of the study indicates a high potential of PEG·SPIONs in medical MRI.
聚乙二醇（PEG）包覆超顺磁性氧化铁纳米粒子（PEG·SPIONs）通过简便的一锅法制备。合成的 PEG·SPIONs 粒径均匀，平均水动力直径为 11.7nm。PEG·SPIONs 在水中表现出良好的分散性、胶体稳定性和生物相容性。对 PEG·SPIONs 的磁共振成像（MRI）特性进行了体外和体内研究。T1 和 T2 加权成像的双重对比均得到了很好的增强，纵向弛豫率（r1）和横向弛豫率（r2）分别为 35.92s-1·mM-1Fe(3+)和 206.91s-1·mM-1Fe(3+)。体内 T2 加权 MRI 显示肝脏和脾脏显著增强，而 T1 加权 MRI 则没有增强。研究发现，纳米粒子主要在肝脏、脾脏和肠道积聚，而在肾脏、心脏和肺部的摄取量较低。通过肝胆（HB）处理，在 14 天内观察到 PEG·SPIONs 的逐渐排泄。还在体外和体内评估了 PEG·SPIONs 的毒性。通过对苏木精-伊红染色后的器官组织进行研究，发现 PEG·SPIONs 具有良好的生物相容性。研究结论表明，PEG·SPIONs 在医学 MRI 中有很高的应用潜力。
