Ramos Rogério Rodrigo, Paiva Jeferson Leandro de, Gomes José Paulo Franco Dos Santos, Boer Nagib Pezati, Godoy José Maria Pereira de, Batigalia Fernando
Department of Human Anatomy of Universidade Brasil - Fernandópolis (SP), Brazil.
Clinical Laboratory of the Fundação Educacional de Fernandópolis (FEF-SP) - Fernandópolis (SP), Brazil.
An Bras Dermatol. 2017 Sep-Oct;92(5):622-625. doi: 10.1590/abd1806-4841.20175651.
Photodynamic therapy is a therapeutic modality that has consolidated its activity in the photooxidation of organic matter, which arises from the activity of reactive oxygen species.
To evaluate the effect of red laser 660nm with the photosensitizer methylene blue on Propionibacterium acnes in vitro.
The experimental design was distributed into four groups (1 - control group without the application of light and without photosensitizer, 2 - application of light, 3 - methylene blue without light, and 4 - methylene blue with light). Tests were subjected to red laser irradiation 660nm by four cycles of 5 minutes at 3-minute intervals.
It was evidenced the prominence of the fourth cycle (20 minutes) groups 2, 3 and 4.
Despite the favorable results, the laser irradiation time photosensitizer associated with methylene blue were not sufficient to to completely inhibit the proliferation of bacteria.
Further studies in vitro are recommended to enable the clinical application of this photosensitizer in photodynamic therapy.
光动力疗法是一种在活性氧物种活动产生的有机物光氧化中巩固其活性的治疗方式。
评估660nm红色激光联合光敏剂亚甲蓝对痤疮丙酸杆菌的体外作用。
实验设计分为四组（1 - 不使用光和光敏剂的对照组，2 - 使用光，3 - 无光时使用亚甲蓝，4 - 光联合亚甲蓝）。测试以3分钟的间隔进行四个5分钟周期的660nm红色激光照射。
证实了第2、3和4组在第四个周期（20分钟）时的显著效果。
尽管结果良好，但与亚甲蓝联合使用的激光照射时间不足以完全抑制细菌增殖。
建议进一步进行体外研究，以使这种光敏剂能够在光动力疗法中临床应用。
