Sharkey S W, Elsperger K J, Murakami M, Apple F S
Cardiology Division, Hennepin County Medical Center, Minneapolis, Minnesota.
Am J Physiol. 1989 Feb;256(2 Pt 2):H508-14. doi: 10.1152/ajpheart.1989.256.2.H508.
The response of the myocardial creatine kinase system to acute coronary artery occlusion is not well defined. In the present study, we measured serial changes in myocardial creatine kinase and creatine kinase-MB activities after acute occlusion of the left anterior descending (LAD) coronary artery in 20 open-chest pentobarbital-anesthetized dogs. Tissue samples were obtained from both ischemic and nonischemic left ventricular myocardium at base line and 1-, 3-, and 5-h intervals after LAD occlusion and assayed for total creatine kinase and the isoenzymes creatine kinase-MM, and creatine kinase-MB. Total creatine kinase activity declined significantly in both the ischemic and the nonischemic tissue because of a decline in creatine kinase-MM activity. Concomitantly, creatine kinase-MB activity increased significantly in both the ischemic and the nonischemic tissue. These changes were observed when the duration of the LAD occlusion was 3 h or longer, but not when the duration of the occlusion was 1 h. Thus acute myocardial ischemia causes pronounced changes in the canine myocardial creatine kinase system. These rapid biochemical alterations occur both locally in ischemic tissue and remotely in nonischemic tissue.
心肌肌酸激酶系统对急性冠状动脉闭塞的反应尚不明确。在本研究中，我们测定了20只戊巴比妥麻醉的开胸犬左前降支（LAD）冠状动脉急性闭塞后，心肌肌酸激酶和肌酸激酶-MB活性的系列变化。在基线以及LAD闭塞后1小时、3小时和5小时的间隔时间，从缺血和非缺血的左心室心肌获取组织样本，并检测总肌酸激酶以及肌酸激酶-MM和肌酸激酶-MB同工酶。由于肌酸激酶-MM活性下降，缺血和非缺血组织中的总肌酸激酶活性均显著下降。与此同时，缺血和非缺血组织中的肌酸激酶-MB活性均显著增加。当LAD闭塞持续时间为3小时或更长时间时观察到这些变化，但闭塞持续时间为1小时时未观察到。因此，急性心肌缺血会导致犬心肌肌酸激酶系统发生显著变化。这些快速的生化改变既发生在缺血组织的局部，也发生在非缺血组织的远处。
