Lin J L, Lin W C, Liu J K, Surampalli R Y, Zhang T C, Kao C M
Water Environ Res. 2017 Dec 1;89(12):2113-2121. doi: 10.2175/106143017X15054988926415.
The goals of this study were to assess the effectiveness of (1) enhancing octachlorinated dibenzo-p-dioxin (OCDD) biodegradation under aerobic conditions by Pseudomonas mendocina NSYSU (P. Mendocina NSYSU) with the addition of lecithin, and (2) inducing OCDD ring-cleavage genes by pentachlorophenol (PCP) and OCDD addition. P. Mendocina NSYSU could biodegrade OCDD via aerobic cometabolism and lecithin was used as a primary substrate. Approximately 74 and 67% of OCDD biodegradation was observed after 60 days of incubation with lecithin and glucose supplement, respectively. Lecithin was also used as the solubilization additive resulting in OCDD solubilization and enhanced bioavailability of OCDD to P. Mendocina NSYSU. Two intradiol and extradiol ring-cleavage dioxygenase genes (Pmen_0474 and Pmen_2526) were identified from gene analyses. Gene concentration was significantly enhanced after the inducement by PCP and OCDD. Higher gene inducement efficiency was obtained using PCP as the inducer, and Pmen_2526 played a more important role in OCDD biodegradation.
(1) 通过添加卵磷脂，增强门多萨假单胞菌NSYSU（P. Mendocina NSYSU）在有氧条件下对八氯二苯并 - 对二噁英（OCDD）的生物降解效果；(2) 通过添加五氯苯酚（PCP）和OCDD诱导OCDD环裂解基因。P. Mendocina NSYSU可通过有氧共代谢作用对OCDD进行生物降解，并将卵磷脂用作主要底物。分别在添加卵磷脂和葡萄糖培养60天后，观察到约74%和67%的OCDD发生了生物降解。卵磷脂还用作增溶添加剂，导致OCDD增溶，并提高了OCDD对P. Mendocina NSYSU的生物可利用性。通过基因分析鉴定出两个内二醇和外二醇环裂解双加氧酶基因（Pmen_0474和Pmen_2526）。在PCP和OCDD诱导后，基因浓度显著提高。以PCP作为诱导剂可获得更高的基因诱导效率，且Pmen_2526在OCDD生物降解中发挥更重要的作用。
