Department of Experimental Animal Centre, XuanWu Hospital of Capital Medical University, Beijing, People's Republic of China.
Inflammation. 2018 Mar;41(2):432-436. doi: 10.1007/s10753-017-0699-x.
The aim of this study is to investigate the cardioprotective effects of morroniside in rats following acute myocardial infarction. An acute myocardial infarction (AMI) was induced by ligating the anterior descending coronary artery (LAD) [1]. Following AMI, morroniside was administered intragastrically for 24 h at doses of 45, 90, and 180 mg/kg, respectively. Biomarkers such as creatine kinase (CK-MB), lactate dehydrogenase (LDH), ɑ-hydroxybutyrate dehydrogenase (ɑ-HBDH), and aspartate aminotransferase (AST) activities in AMI rats in the serum were detected with commercial kits [2]. Following AMI, morroniside was administered intragastrically for 72 h at doses of 45, 90, and 180 mg/kg/d, respectively. The expression of nuclear factor kappa B (NF-κB) in cardiac myocardium was detected by western blotting analysis. Meanwhile, cardiac function was measured by echocardiography. We observed morroniside decreased the levels of CK-MB, LDH, ɑ-HBDH, and AST activities in AMI rats after 24 h. We also found that morroniside reduced the expression of NF-κB in cardiac myocardium at 72 h post AMI rats. Further, cardiac function was improved by administration of morroniside. Collectively, our findings demonstrated that morroniside had cardioprotective effects in rats following acute myocardial infarction. Attenuation of inflammation might contribute to the cardioprotective effects of morroniside.
本研究旨在探讨桃叶珊瑚苷在大鼠急性心肌梗死（AMI）后的心脏保护作用。通过结扎前降支冠状动脉（LAD）[1]诱导 AMI。AMI 后，桃叶珊瑚苷分别以 45、90 和 180mg/kg 的剂量灌胃给药 24h。采用商业试剂盒检测 AMI 大鼠血清中肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）、α-羟丁酸脱氢酶（α-HBDH）和天冬氨酸氨基转移酶（AST）等生物标志物的活性[2]。AMI 后，桃叶珊瑚苷分别以 45、90 和 180mg/kg/d 的剂量灌胃给药 72h。采用 Western blot 分析检测心肌细胞核因子κB（NF-κB）的表达。同时，通过超声心动图测量心功能。结果显示，24h 后，桃叶珊瑚苷降低了 AMI 大鼠 CK-MB、LDH、α-HBDH 和 AST 活性。还发现，桃叶珊瑚苷降低了 AMI 大鼠心肌 NF-κB 的表达。进一步的，桃叶珊瑚苷给药改善了心功能。综上所述，本研究结果表明，桃叶珊瑚苷对大鼠急性心肌梗死具有心脏保护作用。抑制炎症可能是桃叶珊瑚苷发挥心脏保护作用的机制之一。
