Department of Neurology, Hanyang University Guri Hospital, 153, Gyeongchun-ro, Guri-si, Gyeonggi-do 11923, South Korea.
Department of Radiology, Hanyang University Guri Hospital, 153, Gyeongchun-ro, Guri-si, Gyeonggi-do 11923, South Korea.
Biomaterials. 2018 Feb;155:80-91. doi: 10.1016/j.biomaterials.2017.11.009. Epub 2017 Nov 14.
In vivo tracking of transplanted stem cells has been a central aim of stem cell therapy. Although many tracking systems have been introduced, no method has yet been validated for clinical applications. We developed a novel sophisticated peptide (GV1001) that mimics hTERT (human telomerase reverse transcriptase) and analysed its ability to track and protect stem cells after transplantation. Ferrocenecarboxylic acid-conjugated GV1001 (Fe-GV1001) efficiently penetrated stem cells with no adverse effects. Moreover, Fe-GV1001 improved the viability, proliferation, and migration of stem cells under hypoxia. After Fe-GV1001-labelled stem cells were transplanted into the brains of rats after stroke, the labelled cells were easily tracked by MRI. Our findings indicate that Fe-GV1001 can be used for the in vivo tracking of stem cells after transplantation into the brain and can improve the efficacy of stem cell therapy by sustaining and enhancing stem cell characteristics under disease conditions.
在体追踪移植干细胞一直是干细胞治疗的核心目标。尽管已经引入了许多追踪系统，但还没有一种方法得到临床应用的验证。我们开发了一种新的复杂肽（GV1001），模拟端粒酶逆转录酶（hTERT），并分析其在移植后追踪和保护干细胞的能力。二茂铁羧酸偶联的 GV1001（Fe-GV1001）能够有效地穿透干细胞，没有不良反应。此外，Fe-GV1001 提高了缺氧条件下干细胞的活力、增殖和迁移能力。在中风后将 Fe-GV1001 标记的干细胞移植到大鼠脑内后，通过 MRI 很容易追踪到标记的细胞。我们的研究结果表明，Fe-GV1001 可用于在体追踪移植到脑内的干细胞，并通过在疾病条件下维持和增强干细胞特性来提高干细胞治疗的疗效。
