Singh A, Reed M J, Ghilchik M W, James V H
Department of Chemical Pathology, St. Mary's Hospital Medical School, London, U.K.
Cancer Lett. 1989 Jan;44(1):45-8. doi: 10.1016/0304-3835(89)90106-7.
We have prepared cytosols of normal breast tissue and breast tumour tissue and examined their effect on 17 beta-oestradiol dehydrogenase (E2DH) activity in cultured normal breast fibroblasts and on the MCF-7 breast cancer cell line. Tumour cytosol increased the activity of E2DH in the reductive (E1----E2) direction in both MCF-7 cells and normal breast fibroblasts. E2DH activity in the oxidative (E2----E1) direction did not change. Increasing the amount of tumour cytosol progressively raised E2DH activity in the E1----E2 direction in MCF-7 cells, whereas equivalent amounts of cytosol from normal tissue had no effect. No change was seen in the oxidative direction. These findings suggest that a factor present in breast tumours may influence E2DH activity and consequently, the hormonal environment and growth of tumours.
我们制备了正常乳腺组织和乳腺肿瘤组织的胞质溶胶，并检测了它们对培养的正常乳腺成纤维细胞和MCF-7乳腺癌细胞系中17β-雌二醇脱氢酶（E2DH）活性的影响。肿瘤胞质溶胶增加了MCF-7细胞和正常乳腺成纤维细胞中E2DH在还原方向（E1→E2）的活性。氧化方向（E2→E1）的E2DH活性没有变化。增加肿瘤胞质溶胶的量可使MCF-7细胞中E2DH在E1→E2方向的活性逐渐升高，而等量的正常组织胞质溶胶则没有影响。氧化方向未见变化。这些发现表明，乳腺肿瘤中存在的一种因子可能影响E2DH活性，进而影响肿瘤的激素环境和生长。
