表皮生长因子、转化生长因子及乳腺肿瘤匀浆对培养脂肪组织中雌二醇17β-羟类固醇脱氢酶活性的影响

The effect of epidermal growth factor, transforming growth factor and breast tumour homogenates on the activity of oestradiol 17 beta hydroxysteroid dehydrogenase in cultured adipose tissue.

作者信息

McNeill J M, Reed M J, Beranek P A, Newton C J, Ghilchik M W, James V H

出版信息

Cancer Lett. 1986 May;31(2):213-9. doi: 10.1016/0304-3835(86)90013-3.

Abstract

The effect of epidermal growth factor (EGF), transforming growth factor (TGF alpha) and breast tumour homogenates on oestradiol 17 beta hydroxysteroid dehydrogenase (E2DH) activity has been examined using cultured human breast adipose tissue. EGF (100-1000 ng/ml) inhibited E2DH activity (E1----E2) in a dose dependent manner. TGF alpha (250 and 500 ng/ml) stimulated E2DH activity, with conversion of E1----E2 increasing to a greater degree than E2----E1 activity. Breast tumour homogenates (2-10% w/v) also influenced E2DH activity. It is concluded that growth factors, produced by breast tumours, may modulate E2DH activity in tissues surrounding the tumour and thereby influence tumour growth.

摘要

利用培养的人乳腺脂肪组织，研究了表皮生长因子（EGF）、转化生长因子（TGFα）和乳腺肿瘤匀浆对雌二醇17β羟类固醇脱氢酶（E2DH）活性的影响。EGF（100 - 1000 ng/ml）以剂量依赖性方式抑制E2DH活性（E1→E2）。TGFα（250和500 ng/ml）刺激E2DH活性，E1→E2的转化增加程度大于E2→E1活性。乳腺肿瘤匀浆（2 - 10% w/v）也影响E2DH活性。得出的结论是，乳腺肿瘤产生的生长因子可能调节肿瘤周围组织中的E2DH活性，从而影响肿瘤生长。