Xiang Bin, Liang Jianpeng, You Renrong, Han Lujie, Mei Kun, Chen Libin, Chen Ruojing, Zhang Yifan, Dai Xu, Gao Pei, Liao Ming, Xiao Chencheng, Ren Tao
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, China; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, China; College of Animal Science and Technology, Shihezi University, China.
Vet Microbiol. 2017 Dec;212:16-21. doi: 10.1016/j.vetmic.2017.10.022. Epub 2017 Nov 2.
Since the first outbreak of H5N6 reported in Laos at 2013, there has been a dramatic increase in H5N6 strains isolated from waterfowl in China, particularly Southern China. However, pathogenicity and transmissibility of the virus in different birds remain largely unknown. In this study, a novel H5N6 virus, termed QY01, that belonged to group C in 2.3.4.4 was isolated from an apparently healthy domestic goose in Guangdong province, southern China in 2016. In order to simulate the natural transmission of different kinds of birds, we evaluated its pathogenicity and transmissibility in chickens, domestic geese and pigeons. To investigate the replication and shedding of QY01 in poultry, chickens, geese and pigeons were inoculated intranasally with 10 EID of virus. In addition, to measure intra-species transmission of QY01, three sentinel birds were housed with each group. The results demonstrated that QY01 exhibited a highly pathogenic phenotype, and was transmissible among in chickens and geese. However, the virus did not appear to be pathogenic in pigeons, indicating that this novel H5N6 virus exhibited different host ranges and tissue tropisms, and may pose a substantial risk for the chicken and goose industry. Therefore, continued surveillance for H5N6 AIVs is necessary, and increased attention should be paid to cross-species transmission between waterfowl and terrestrial birds.
自2013年老挝首次报告H5N6疫情以来，中国尤其是中国南方从水禽中分离出的H5N6毒株急剧增加。然而，该病毒在不同禽类中的致病性和传播性仍 largely unknown。在本研究中，2016年从中国南方广东省一只看似健康的家鹅中分离出一种新型H5N6病毒，命名为QY01，它属于2.3.4.4分支中的C组。为了模拟不同禽类的自然传播，我们评估了其在鸡、家鹅和鸽子中的致病性和传播性。为了研究QY01在家禽中的复制和排毒情况，将鸡、鹅和鸽子经鼻接种10 EID的病毒。此外，为了检测QY01的种内传播，每组饲养三只哨兵鸟。结果表明，QY01表现出高致病性表型，可在鸡和鹅之间传播。然而，该病毒在鸽子中似乎没有致病性，这表明这种新型H5N6病毒表现出不同的宿主范围和组织嗜性，可能对鸡和鹅产业构成重大风险。因此，有必要继续监测H5N6禽流感病毒，并应更加关注水禽与陆禽之间的跨物种传播。
