婴幼儿体外循环期间围手术期血浆线粒体DNA动态变化及其与炎症的相关性
Perioperative plasma mitochondrial DNA dynamics and correlation with inflammation during infantile cardiopulmonary bypass.
作者信息
Xu Fei, Liu Rui-Qi, Cao Rong, Guo Lang-Tao, Zhang Ning, Huang Ke, Cui Yu, Li Wei-Na, Li Lei, Huang Zheng-Hua
机构信息
Department of Anesthesiology, Chengdu Women and Children's Central Hospital, Chongqing Medical University, Chengdu, Sichuan 610091, China.
Department of Burns and Plastic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Indian Heart J. 2017 Nov-Dec;69(6):797-800. doi: 10.1016/j.ihj.2017.03.009. Epub 2017 Mar 28.
OBJECTIVE
Numerous studies in animals and humans have demonstrated that inflammatory mediators such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8 play a role in cardiopulmonary bypass (CPB), which might affect surgical outcomes. Plasma mitochondrial DNA (mtDNA), a recently discovered pro-inflammatory agent, is released by cells upon insult. This study aimed to detect changes in plasma mtDNA levels at different time points after infantile CPB and explore its potential association with inflammatory mediators.
METHODS
In the present study, we analyzed the perioperative plasma mtDNA and inflammatory cytokine levels of 48 infants undergoing ventricular septal defect closure. Blood samples were collected before aortic cross-clamping (T1), at the end of CPB (T2), and 6h (T3), 12h (T4), and 24h (T5) post-CPB. Reverse transcription-polymerase chain reaction and specific enzyme-linked immunosorbent assay were used to quantify the plasma mtDNA and inflammatory cytokines, respectively. Bivariate correlation analysis was used to determine the correlations between plasma mtDNA and inflammatory cytokines.
RESULTS
Plasma mtDNA levels increased at T2 and peaked at T3. Significant positive correlations were found between peak plasma mtDNA (at T3) and several inflammatory biomarkers, including IL-6 (at T3) (r=0.62, P<0.001), IL-8 (at T2) (r=0.53, P<0.001), and TNF-α (at T3) (r=0.61, P<0.001).
CONCLUSION
Here we report that mtDNA may participate in a systemic inflammatory response to CPB.
目的
众多动物和人体研究表明，肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6和IL-8等炎症介质在体外循环（CPB）中发挥作用，这可能会影响手术结果。血浆线粒体DNA（mtDNA）是一种最近发现的促炎因子，在细胞受到损伤时会被释放出来。本研究旨在检测婴儿CPB后不同时间点血浆mtDNA水平的变化，并探讨其与炎症介质的潜在关联。
方法
在本研究中，我们分析了48例接受室间隔缺损修补术婴儿的围手术期血浆mtDNA和炎症细胞因子水平。在主动脉阻断前（T1）、CPB结束时（T2）以及CPB后6小时（T3）、12小时（T4）和24小时（T5）采集血样。分别采用逆转录聚合酶链反应和特异性酶联免疫吸附测定法对血浆mtDNA和炎症细胞因子进行定量分析。采用双变量相关分析确定血浆mtDNA与炎症细胞因子之间的相关性。
结果
血浆mtDNA水平在T2时升高，并在T3时达到峰值。血浆mtDNA峰值（T3时）与几种炎症生物标志物之间存在显著正相关，包括IL-6（T3时）（r = 0.62，P < 0.001）、IL-8（T2时）（r = 0.53，P < 0.001）和TNF-α（T3时）（r = 0.61，P < 0.001）。
结论
我们在此报告，mtDNA可能参与了对CPB的全身炎症反应。
Zotero 插件