Shalom Ohad, Adler Amos
Clinical Microbiology Laboratory, Tel Aviv Sourasky Medical Center, Israel.
Clinical Microbiology Laboratory, Tel Aviv Sourasky Medical Center, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Diagn Microbiol Infect Dis. 2018 Feb;90(2):73-76. doi: 10.1016/j.diagmicrobio.2017.10.008. Epub 2017 Oct 19.
The goals of the study were to examine the analytic and performance parameters of 2 commercial rapid carbapenem-hydrolysis assays, the β-CARBA test (Bio-Rad) and the Rapid CARB Blue Kit (ROSCO) in comparison with an in-house CARBA NP assay for the detection of Carbapenemase-Producing Enterobacteriaceae (CPE). Their performance was evaluated following growth on 2 chromogenic CPE screening media. The sensitivity was highest (91%) in the β-CARBA test when used from the mSuperCARBA™ plates and was lowest when the same test was used from the chromID™ CARBA plates (75%). The specificity was highest in the NP CARBA test in both media (96%), followed by the β CARBA/mSuperCARBA™ combination (92%). The specificity of the Rapid CARB Blue Kit was as low as 36% when used with the chromID™ CARBA plates. The β-CARBA test was simple to use and had the shortest turn-around time and hand-on time.
本研究的目的是检测两种商业化快速碳青霉烯水解试验（β-CARBA试验，Bio-Rad公司；快速CARB蓝色试剂盒，ROSCO公司）的分析参数和性能参数，并与一种用于检测产碳青霉烯酶肠杆菌科细菌（CPE）的自制CARBA NP试验进行比较。在两种显色CPE筛选培养基上生长后，对它们的性能进行了评估。当使用mSuperCARBA™平板进行β-CARBA试验时，灵敏度最高（91%）；而当使用chromID™ CARBA平板进行相同试验时，灵敏度最低（75%）。在两种培养基中，NP CARBA试验的特异性最高（96%），其次是β CARBA/mSuperCARBA™组合（92%）。当与chromID™ CARBA平板一起使用时，快速CARB蓝色试剂盒的特异性低至36%。β-CARBA试验使用简便，周转时间和操作时间最短。
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