Song Liang-Li, Cui Yan, Yu Si-Jiu, Liu Peng-Gang, Liu Jun, Yang Xue, He Jun-Feng, Zhang Qian
Laboratory of Animal Anatomy & Tissue Embryology, Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China.
Laboratory of Animal Anatomy & Tissue Embryology, Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China; Gansu Province Livestock Embryo Engineering Research Center, Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China.
Gen Comp Endocrinol. 2018 May 1;260:18-24. doi: 10.1016/j.ygcen.2017.11.016. Epub 2017 Nov 22.
Bone morphogenetic protein 2 (BMP2), BMP receptor-IA (BMPR-IA), and the BMP2 antagonist Noggin are important proteins involved in regulating the hair follicle (HF) cycle in skin. In order to explore the expression profiles of BMP2, BMPR-IA, and Noggin in the HF cycle of yak skin, we collected adult yak skin in the telogen, proanagen, and midanagen phases of HFs and evaluated gene and protein expression by real-time quantitative polymerase chain reaction (qRT-PCR), western blotting, and immunohistochemistry. qRT-PCR and western blotting results showed that BMP2 and BMPR-IA expression levels were highest in the telogen of HFs and higher than that of Noggin in the same phase. The expression of Noggin was significantly higher in proanagen and midanagen phases of HFs than in the telogen phase, with the highest expression observed in the proanagen phase. Moreover, the expression of Noggin in the proanagen phase was significantly higher than those of BMP2 and BMPR-IA during the same phase. Immunohistochemistry results showed that BMP2, BMPR-IA, and Noggin were expressed in the skin epidermis, sweat glands, sebaceous glands, HF outer root sheath, and hair matrix. In summary, the characteristic expression profiles of BMP2, BMPR-IA, and Noggin suggested that BMP2 and BMPR-IA had inhibitory effects on the growth of HFs in yaks, whereas Noggin promoted the growth of yak HFs, mainly by affecting skin epithelial cell activity. These results provide a basis for further studies of HF development and cycle transition in yak skin.
骨形态发生蛋白2（BMP2）、BMP受体-IA（BMPR-IA）和BMP2拮抗剂头蛋白（Noggin）是参与调节皮肤毛囊（HF）周期的重要蛋白质。为了探究BMP2、BMPR-IA和Noggin在牦牛皮肤HF周期中的表达谱，我们收集了处于HF静止期、生长期前期和生长期中期的成年牦牛皮肤，并通过实时定量聚合酶链反应（qRT-PCR）、蛋白质印迹法和免疫组织化学评估基因和蛋白质表达。qRT-PCR和蛋白质印迹法结果显示，BMP2和BMPR-IA的表达水平在HF静止期最高，且在同一阶段高于Noggin。Noggin在HF生长期前期和生长期中期的表达明显高于静止期，在生长期前期观察到最高表达。此外，生长期前期Noggin的表达明显高于同一阶段的BMP2和BMPR-IA。免疫组织化学结果显示，BMP2、BMPR-IA和Noggin在皮肤表皮、汗腺、皮脂腺、HF外根鞘和毛基质中表达。总之，BMP2、BMPR-IA和Noggin的特征性表达谱表明，BMP2和BMPR-IA对牦牛HF的生长具有抑制作用，而Noggin主要通过影响皮肤上皮细胞活性促进牦牛HF的生长。这些结果为进一步研究牦牛皮肤HF发育和周期转换提供了依据。
