Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
Res Vet Sci. 2018 Apr;117:54-56. doi: 10.1016/j.rvsc.2017.11.006. Epub 2017 Nov 11.
PB2-627K is an important amino acid that determines the virulence of some influenza A viruses. However, it has not been experimentally investigated in the H3N2 swine influenza virus. To explore the potential role of PB2-K627E substitution in H3N2 swine influenza virus, the growth properties and pathogenicity between H3N2 swine influenza virus and its PB2-K627E mutant were compared. For the first time, our results showed that PB2-K627E mutation attenuates H3N2 swine influenza virus in mammalian cells and in mice, suggesting that PB2-627K is required for viral replication and pathogenicity of H3N2 swine influenza virus.
PB2-627K 是决定某些甲型流感病毒毒力的重要氨基酸。然而，在 H3N2 猪流感病毒中尚未进行实验研究。为了探索 PB2-K627E 取代在 H3N2 猪流感病毒中的潜在作用，比较了 H3N2 猪流感病毒与其 PB2-K627E 突变体的生长特性和致病性。我们的研究结果首次表明，PB2-K627E 突变使 H3N2 猪流感病毒在哺乳动物细胞和小鼠中减毒，提示 PB2-627K 是 H3N2 猪流感病毒复制和致病性所必需的。
