Castro Natália A, Pfeifer Luiz F M, Andrade Jéssica S, Rincón Joao A A, Pegoraro Ligia M Cantarelli, Schneider Augusto
Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Empresa Brasileira de Pesquisa Agropecuária Embrapa - Rondônia, Porto Velho, RO, Brazil.
Anim Reprod Sci. 2018 Jan;188:130-136. doi: 10.1016/j.anireprosci.2017.11.017. Epub 2017 Nov 22.
Paraoxonase-1 (PON1) activity has been associated with improvement in ovarian function in early postpartum dairy cows and improved in vitro embryo development. The aim of the current study was to evaluate the potential association among PON1 activity and follicular growth, diameter of the preovulatory follicle and pregnancy per artificial insemination (AI) service in cattle. In Experiment 1, cows (n=33) were subjected to an estradiol-progesterone based protocol to control time of ovulation. Starting on Day 8 of the protocol, follicular growth and serum PON1 activity were monitored. Cows were separated according to the occurrence of ovulation into two groups: Ovulatory (Ov; n=22) and Anovulatory (Anov; n=11). The serum activity of PON1 was not different between Ov and Anov cows (P=0.94). In addition, using a regression model there was no effect of serum PON1 activity on the diameter of dominant follicle (r=0.00; P=0.99). In Experiment 2, cows (n=193) were submitted to the same hormonal protocol as in Experiment 1. On the day of the timed artificial insemination (TAI), the diameter of dominant follicle was evaluated and blood samples were collected for analysis of PON1 activity. According to the serum PON1 activity, cows were divided into three groups: Low (<70U/mL), Medium (70-90U/mL) or High (>90U/mL) PON1 activity. The overall pregnancy rate was 62.7% (121/193), with no difference among PON1 activity groups. Additionally, using a regression model there was no effect of serum PON1 activity on the diameter of the preovulatory follicle (r=0.03; P=0.65) and pregnancy rate (r=0.005; P=0.94). The results of this study indicate that there is no effect of serum PON1 activity on the diameter of preovulatory follicle or establishment of pregnancy in cows submitted to time of ovulation synchronization protocols.
对氧磷酶-1（PON1）活性与产后早期奶牛卵巢功能的改善以及体外胚胎发育的改善有关。本研究的目的是评估牛体内PON1活性与卵泡生长、排卵前卵泡直径以及人工授精（AI）后的妊娠之间的潜在关联。在实验1中，对33头奶牛采用基于雌二醇-孕酮的方案来控制排卵时间。从方案的第8天开始，监测卵泡生长和血清PON1活性。根据排卵情况将奶牛分为两组：排卵组（Ov；n = 22）和不排卵组（Anov；n = 11）。排卵组和不排卵组奶牛的血清PON1活性没有差异（P = 0.94）。此外，使用回归模型分析发现，血清PON1活性对优势卵泡直径没有影响（r = 0.00；P = 0.99）。在实验2中，193头奶牛采用与实验1相同的激素方案。在定时人工授精（TAI）当天，评估优势卵泡直径并采集血样分析PON1活性。根据血清PON1活性，将奶牛分为三组：低（<70U/mL）、中（70 - 90U/mL）或高（>90U/mL）PON1活性组。总体妊娠率为62.7%（121/193），PON1活性组之间没有差异。此外，使用回归模型分析发现，血清PON1活性对排卵前卵泡直径（r = 0.03；P = 0.65）和妊娠率（r = 0.005；P = 0.94）没有影响。本研究结果表明，对于采用排卵同步方案的奶牛，血清PON1活性对排卵前卵泡直径或妊娠的建立没有影响。
