Murakami Daigo, Takamori Shinzo, Kawahara Akihiko, Mitsuoka Masahiro, Kashihara Masaki, Yoshiyama Koichi, Matsumoto Ryoichi, Yokoyama Shintaro, Fujimoto Kiminori, Kawaguchi Atsushi, Izuhara Kenji, Akagi Yoshito
Department of Surgery, Kurume University School of Medicine.
Department of Diagnostic Pathology, Kurume University Hospital.
Kurume Med J. 2018 Feb 26;64(1.2):13-20. doi: 10.2739/kurumemedj.MS640012. Epub 2017 Nov 27.
Periostin is an extracellular matrix N-glycoprotein that is a major constituent of the desmoplastic stroma around solid tumors. Periostin promotes tumor invasion and metastasis via epithelial-mesenchymal transition. The aims of this study were to evaluate periostin expression immunohistochemically and quantitatively in patients with non-small cell lung cancer (NSCLC) and to assess any associations with clinical features and prognosis. A total of 184 specimens of NSCLC tissue were investigated, including 134 adenocarcinomas, 39 squamous cell carcinomas, and 11 other histologic subtypes. The intra-tumoral periostin expression area in each captured field was calculated using the image processing integration software WinROOF. The mean periostin expression score was classified as high or low by the median value of its expression area. Univariate analysis demonstrated that gender, tumor size, T status, N status, stage, histologic type, smoking habits, percent vital capacity, 1% forced expiratory volume, and pleural invasion were each significantly associated with periostin scores. Multivariate analysis revealed that high periostin expression score was an independent prognostic factor significantly associated with decreased cancer-specific survival (HR, 3.65; 95% CI, 1.04-12.84; P=0.0439). We concluded that intratumoral periostin expression was an independent prognostic factor for NSCLC.
骨膜蛋白是一种细胞外基质N-糖蛋白，是实体瘤周围促结缔组织增生性基质的主要成分。骨膜蛋白通过上皮-间质转化促进肿瘤侵袭和转移。本研究的目的是通过免疫组织化学和定量方法评估非小细胞肺癌（NSCLC）患者的骨膜蛋白表达，并评估其与临床特征和预后的相关性。共研究了184例NSCLC组织标本，包括134例腺癌、39例鳞状细胞癌和11例其他组织学亚型。使用图像处理集成软件WinROOF计算每个采集视野内肿瘤内骨膜蛋白表达面积。根据其表达面积的中位数将骨膜蛋白平均表达评分分为高或低。单因素分析表明，性别、肿瘤大小、T分期、N分期、阶段、组织学类型、吸烟习惯、肺活量百分比、第1秒用力呼气量和胸膜侵犯均与骨膜蛋白评分显著相关。多因素分析显示，骨膜蛋白高表达评分是与癌症特异性生存率降低显著相关的独立预后因素（HR，3.65；95%CI，1.04-12.84；P=0.0439）。我们得出结论，肿瘤内骨膜蛋白表达是NSCLC的独立预后因素。
