Shi Liyang, Zhang Yu, Ossipov Dmitri
Science for Life Laboratory, Division of Polymer Chemistry, Department of Chemistry-Ångström, Uppsala University, Uppsala, Sweden.
College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi, 710069, People's Republic of China.
Biopolymers. 2018 Feb;109(2). doi: 10.1002/bip.23090. Epub 2017 Nov 27.
In situ cross-linked hyaluronan (HA) hydrogels with different capacities for biomineralization were prepared and their enzymatic degradation was monitored. Covalent incorporation of bisphosphonates (BPs) into HA hydrogel results in the increased stiffness of the hydrogel in comparison with the unmodified HA hydrogel of the same cross-linking density. The rate of enzymatic degradation of HABP hydrogel was significantly lower than the rate of degradation of control HA hydrogel in vitro. This effect is observed only in the presence of calcium ions that strongly bind to the matrix-anchored BP groups and promote further mineralization of the matrix. The degradation of the hydrogels was followed by noninvasive fluorescence measurements enabled after mild and chemoselective labeling of cross-linkable HA derivatives with a fluorescent tag.
制备了具有不同生物矿化能力的原位交联透明质酸（HA）水凝胶，并监测了它们的酶促降解情况。与相同交联密度的未修饰HA水凝胶相比，双膦酸盐（BPs）共价掺入HA水凝胶会导致水凝胶硬度增加。在体外，HABP水凝胶的酶促降解速率显著低于对照HA水凝胶的降解速率。仅在存在与基质锚定的BP基团强烈结合并促进基质进一步矿化的钙离子时才观察到这种效应。在用荧光标签对可交联HA衍生物进行温和且化学选择性标记后，通过非侵入性荧光测量跟踪水凝胶的降解过程。
