Zhao X
Mentor Biopolymers Ltd, Heriot Watt Research Park, Edinburgh EH14 4AP, UK.
J Biomater Sci Polym Ed. 2006;17(4):419-33. doi: 10.1163/156856206776374115.
Hyaluronic acid (hyaluronan, HA) has many medical applications as a biomaterial. To enhance its biostability, a novel hydrogel of cross-linked hyaluronic acid was prepared using a double cross-linking process, which involves building cross-linkages between hydroxyl group pairs and carboxyl group pairs. The present study explored a number of cross-linking processes in order to obtain different degrees of cross-linking, which were evaluated by the measurement of water absorption capacity as an index of the gel network density. To gain a better understanding of the stability of the gel, the chemical structure and particularly the rheological behaviour of the cross-linked HA, which included the influences of factors, such as degree of cross-linking, HA concentration and gel particle size, were investigated. The in vitro biostability against hyaluronidase and free radical degradation was tested to show that the cross-linked hydrogel had improved resistance to in vitro hyaluronidase and free radical degradation.
透明质酸（又称玻璃酸，HA）作为一种生物材料，在医学领域有诸多应用。为提高其生物稳定性，采用双重交联工艺制备了一种新型的交联透明质酸水凝胶，该工艺涉及在羟基对和羧基对之间建立交联。本研究探索了多种交联工艺以获得不同程度的交联，通过测量吸水能力作为凝胶网络密度的指标来评估交联程度。为更好地理解凝胶的稳定性，研究了交联透明质酸的化学结构，特别是流变行为，其中包括交联度、透明质酸浓度和凝胶粒径等因素的影响。测试了其对透明质酸酶和自由基降解的体外生物稳定性，结果表明交联水凝胶对体外透明质酸酶和自由基降解具有更高的抗性。
