银、二氧化钛和铁纳米颗粒的抗菌及抗生物膜活性。
Antimicrobial and antibiofilm activity of silver, titanium dioxide and iron nano particles.
作者信息
Lavaee Fatemeh, Faez Kiana, Faez Kioumars, Hadi Nahal, Modaresi Farzan
机构信息
Oral and Dental Disease Research Center, School of Dentistry, Department of Oral and Maxillofacial Disease, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
Student Research Committee, School of Dentistry, Department of Oral and Maxillofacial Disease, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Am J Dent. 2016 Dec;29(6):315-320.
PURPOSE
To compare the antimicrobial effects of chlorhexidine, penicillin, erythromycin, clindamycin, tetracycline and vancomycin with silver, titanium dioxide and iron nanoparticles and also to consider the synergistic antibacterial and antibiofilm effects of nanoparticles in clinical and standard strains of Streptococcus mutans and Streptococcus sanguinis.
METHODS
The specimens collected from 66 3-5 year-old children with detected S. mutans and S. sanguinis by PCR were then exposed to the antimicrobial activity of chlorhexidine, penicillin, erythromycin, clindamycin, tetracycline and vancomycin with silver, titanium dioxide and iron nanoparticles measured by microdilution and disc diffusion tests and the colony counted after 1 to 5 minutes. The antibiofilm activity was examined by microtiter test.
RESULTS
Use of nanoparticles alone showed higher minimum inhibitory concentration (MIC) than using them synergistically. The most effective synergistic solution was the one containing TiO2, Ag and Fe3O4 showing 0.019 μg#47;ml in S. mutans and S. sanguinis. Furthermore, this solution had the lowest biofilm inhibitory concentration (BIC) and colony forming units than the other antibiotics and chlorhexidine.
CLINICAL SIGNIFICANCE
The solution containing TiO2, Ag and Fe33O4 showed the lowest inhibitory and antibiofilm concentration against S. mutans and S. sanguinis compared to those of other nanoparticle containing solutions, antibiotics and chlorhexidine, thus it may be used for treating dental caries, dental plaque and oral infections.
目的
比较氯己定、青霉素、红霉素、克林霉素、四环素和万古霉素与银、二氧化钛和铁纳米颗粒的抗菌效果，并探讨纳米颗粒在变形链球菌和血链球菌临床菌株及标准菌株中的协同抗菌和抗生物膜作用。
方法
收集66名3至5岁经PCR检测出变形链球菌和血链球菌的儿童的样本，然后通过微量稀释法和纸片扩散法检测氯己定、青霉素、红霉素、克林霉素、四环素和万古霉素与银、二氧化钛和铁纳米颗粒的抗菌活性，并在1至5分钟后计数菌落。通过微量滴定试验检测抗生物膜活性。
结果
单独使用纳米颗粒时的最低抑菌浓度（MIC）高于协同使用时。最有效的协同溶液是含有TiO2、Ag和Fe3O4的溶液，在变形链球菌和血链球菌中显示为0.019μg/ml。此外，该溶液的生物膜抑制浓度（BIC）和菌落形成单位低于其他抗生素和氯己定。
临床意义
与其他含纳米颗粒溶液、抗生素和氯己定相比，含有TiO2、Ag和Fe3O4的溶液对变形链球菌和血链球菌的抑菌和抗生物膜浓度最低，因此可用于治疗龋齿、牙菌斑和口腔感染。
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