Hatakeyama Daniela M, de Morais-Zani Karen, Serino-Silva Caroline, Grego Kathleen F, Sant'Anna Savio S, Fernandes Wilson, Aniz Patrícia A E A, Torquato Ricardo J S, Tanaka Aparecida S, Sanz Libia, Calvete Juan J, Tanaka-Azevedo Anita M
Laboratório de Herpetologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, Brazil.
Laboratório de Bacteriologia, Instituto Butantan, Av. Vital Brazil, 1500, 05503-900, São Paulo, Brazil.
Toxicon. 2018 Jan;141:34-42. doi: 10.1016/j.toxicon.2017.11.011. Epub 2017 Nov 24.
The number of snakes donated to the Brazilian Instituto Butantan has been decreasing in the past 10 years. This circumstance motivated us to compare the properties of five venom pools of Bothrops jararaca snake stored for up to 54 years. Results showed differences among venom pools regarding enzymatic and other biological activities, such as caseinolytic, phospholipase A, hemorrhagic and coagulant activities, as well as antigenicity. Protein content, reverse-phase chromatographic profile, and immunorecognition by commercial Bothrops antivenom were comparable for all venom pools, although lethality of the most recent preparations was higher. Since the lowest functional activities did not always correspond to older venoms, differences among venom pools used for antivenom production during the period 1963-2008 may correlate with the different proportions of venoms from different localities used in their generation, rather than to long-term storage. We conclude that B. jararaca venoms properly stored for long periods of time retain their structural and pharmacological activities, thus representing useful materials for scientific research and antivenom production.
在过去10年里，捐赠给巴西布坦坦研究所的蛇的数量一直在减少。这种情况促使我们比较了储存长达54年的5个巴西矛头蝮蛇毒池的特性。结果显示，不同毒池的酶活性和其他生物活性存在差异，如酪蛋白溶解活性、磷脂酶A活性、出血活性和凝血活性以及抗原性。所有毒池的蛋白质含量、反相色谱图谱以及商业巴西矛头蝮抗蛇毒血清的免疫识别情况相当，尽管最新制剂的致死性更高。由于最低的功能活性并不总是与较陈旧的毒液相对应，1963年至2008年期间用于抗蛇毒血清生产的不同毒池之间的差异，可能与生产过程中使用的来自不同产地的毒液比例不同有关，而非与长期储存有关。我们得出结论，妥善长期储存的巴西矛头蝮蛇毒保留了其结构和药理活性，因此是科学研究和抗蛇毒血清生产的有用材料。
