Amthor F R, Takahashi E S, Oyster C W
Department of Psychology, School of Optometry, University of Alabama, Birmingham 35294.
J Comp Neurol. 1989 Feb 1;280(1):72-96. doi: 10.1002/cne.902800107.
Rabbit retinal ganglion cells with concentric receptive fields were intracellularly recorded and stained in the isolated superfused eyecup preparation to relate specific physiological response properties to dendritic morphology. Concentric ganglion cells, as traditionally defined, were those that had On or Off centers with antagonistic surrounds but lacked complex response properties such as direction or orientation selectivity. Concentric cells were classified into different groups by extracellular recordings of their On- or Off-center response sign, excitatory receptive field center size, linearity of spatial summation, and brisk vs. sluggish and transient vs. sustained responses to step changes in light intensity. The cells were then impaled, confirmed in identity during intracellular recording, and iontophoretically injected with horseradish peroxidase for histological analysis. Twenty-three concentric ganglion cells were recovered and morphometrically analyzed. Their physiological response properties were found to be related to a number of underlying two- and three-dimensional attributes of the cell's dendritic branching patterns. The dendrites of all 20 brisk concentric cells and two of the three sluggish cells were found to ramify narrowly in either the proximal or distal half of the inner plexiform layer, corresponding to whether they are On center or Off center, respectively. One of the sluggish concentric cells was found to have a more complex, partially bistratified ramification. Physiologically identified brisk-sustained-linear, brisk-transient-nonlinear, brisk-transient-linear, and at least two classes of sluggish concentric ganglion cells were stained. Each of these physiological classes appears to exhibit a distinct and identifiable dendritic branching pattern.
在分离的灌注眼杯标本中，对具有同心感受野的兔视网膜神经节细胞进行细胞内记录和染色，以将特定的生理反应特性与树突形态联系起来。传统定义的同心神经节细胞是那些具有开中心或闭中心以及拮抗周边但缺乏方向或取向选择性等复杂反应特性的细胞。通过细胞外记录同心细胞的开中心或闭中心反应信号、兴奋性感受野中心大小、空间总和的线性以及对光强度阶跃变化的快速与缓慢、瞬态与持续反应，将其分为不同组。然后将细胞刺入，在细胞内记录期间确认其身份，并通过离子电泳注入辣根过氧化物酶进行组织学分析。回收了23个同心神经节细胞并进行了形态计量分析。发现它们的生理反应特性与细胞树突分支模式的许多潜在二维和三维属性有关。发现所有20个快速同心细胞以及3个缓慢细胞中的2个的树突在内部神经丛层的近端或远端一半中狭窄地分支，分别对应于它们是开中心还是闭中心。发现其中一个缓慢同心细胞具有更复杂的、部分双分层的分支。对生理上确定的快速持续线性、快速瞬态非线性、快速瞬态线性以及至少两类缓慢同心神经节细胞进行了染色。这些生理类别中的每一类似乎都表现出独特且可识别的树突分支模式。
