Amthor F R, Takahashi E S, Oyster C W
Department of Psychology, School of Optometry, University of Alabama, Birmingham 35294.
J Comp Neurol. 1989 Feb 1;280(1):97-121. doi: 10.1002/cne.902800108.
Ganglion cells that had complex receptive field properties, namely, On-Off and On direction-selective cells, orientation-selective cells, local edge detectors, and uniformity detectors (suppressed by contrast cells) were recorded in an isolated superfused rabbit eyecup preparation. Cells were first classified by their characteristic extracellular responses to manually controlled stimuli similar to those which have been used in previous in vivo studies. Ganglion cells were then impaled, confirmed in identity by intracellular recording, and iontophoretically injected with horseradish peroxidase for staining. Twenty-two ganglion cells, which included members of all the major classes mentioned above, were recovered from the visual streak or near periphery. All recovered cells were drawn in camera lucida from flat-mounted retinas and entered into a computer as two-dimensional stick figures; nearly all were three-dimensionally reconstructed to determine the level and manner of dendritic ramification in the inner plexiform layer (IPL). The location of ganglion cell dendrites in sublaminar regions of the IPL was found to be consistent with the hypothesis of a division of the IPL into excitatory On (proximal) and Off (distal) sublaminae, with some qualifications for particular classes. Each of the complex receptive field ganglion cell classes exhibited a distinctive three-dimensional dendritic arborization pattern uniquely associated with that physiological class.
在分离的灌注兔眼杯制备物中记录到具有复杂感受野特性的神经节细胞，即开-关和方向选择性开细胞、方向选择性细胞、局部边缘检测器和均匀性检测器（被对比细胞抑制）。细胞首先根据其对人工控制刺激的特征性细胞外反应进行分类，这些刺激类似于先前体内研究中使用的刺激。然后刺入神经节细胞，通过细胞内记录确认其身份，并通过离子电渗法注入辣根过氧化物酶进行染色。从视觉条纹或其附近区域回收了22个神经节细胞，其中包括上述所有主要类型的细胞。所有回收的细胞都用明视野显微镜从扁平视网膜上绘制下来，并作为二维简笔画输入计算机；几乎所有细胞都进行了三维重建，以确定其在内网状层（IPL）中的树突分支水平和方式。发现神经节细胞树突在IPL亚层区域的位置与将IPL分为兴奋性开（近端）和关（远端）亚层的假设一致，但某些特定类型有一些限定条件。每种具有复杂感受野的神经节细胞类型都表现出一种独特的三维树突分支模式，这种模式与该生理类型独特相关。
