Department of Zoology, Faculty of Science, Olabisi Onabanjo University, Ago- Iwoye, Ogun State, Nigeria.
Department of Pure and Applied Zoology, College of Bioscience, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Environ Sci Pollut Res Int. 2018 Feb;25(5):4309-4319. doi: 10.1007/s11356-017-0786-4. Epub 2017 Nov 27.
The increase in the frequency of exposure to gasoline fumes and the growing incidence of infertility among humans has been a major concern and subject of discussion over the years in Nigeria. We therefore present the reproductive effect of gasoline fumes on inhalation exposure in 40 male albino rats. The rats were randomized into five experimental treatments (T) with eight rats per treatment. T1 (control) was exposed to distilled water while T2, T3, T4, and T5 were exposed to gasoline fumes in exposure chambers for 1, 3, 5, and 9 h daily respectively for 12 weeks. Serum level of testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin, oxidative stress markers in the testicular tissue, epididymal sperm health assessment, and testicular histopathology of the rats were used as a diagnostic marker of reproductive dysfunction. Significant (p < 0.05) alterations in the levels of all the reproductive hormones and oxidative stress markers assayed were observed in rats exposed to gasoline fume. Significant reductions (p < 0.05) in sperm count and percentage motility in the exposed rats were observed. Significant (p < 0.05) increased in abnormal sperm cells characterized by damaged head, bent tail, damaged tail, and without head were also observed in the exposed rats. Histopathologically, severe degenerative testicular architectural lesions characterized by alterations in all the generations of sperm cells and reduction of interstitial cells were seen in the exposed rats. Gasoline fume is thus said to interfere with spermatogenesis and impair fertility in male gonad.
在尼日利亚，人们越来越关注汽油烟雾暴露频率的增加和人类不孕不育发病率的上升，这是多年来的主要关注点和讨论话题。因此，我们研究了吸入汽油烟雾对雄性白化大鼠生殖的影响。将 40 只雄性白化大鼠随机分为五组实验处理（T），每组 8 只。T1（对照组）暴露于蒸馏水，而 T2、T3、T4 和 T5 则分别在暴露室内每天暴露于汽油烟雾 1、3、5 和 9 小时，持续 12 周。血清睾酮、卵泡刺激素（FSH）、黄体生成素（LH）、催乳素、睾丸组织中的氧化应激标志物、附睾精子健康评估和大鼠睾丸组织病理学用作生殖功能障碍的诊断标志物。暴露于汽油烟雾的大鼠的所有生殖激素和氧化应激标志物的水平均发生显著（p<0.05）改变。暴露组的精子计数和精子活动率显著降低（p<0.05）。暴露组的异常精子细胞数量也显著增加，表现为头部受损、尾部弯曲、尾部受损和无头。组织病理学检查显示，暴露组的睾丸结构严重退化，所有精子细胞代都发生变化，间质细胞减少。因此，汽油烟雾被认为会干扰精子发生并损害雄性性腺的生育能力。
