大黄素减轻胰腺腺泡AR42J细胞的细胞损伤和炎症。
Emodin attenuates cell injury and inflammation in pancreatic acinar AR42J cells.
作者信息
Zhao Jia-Yu, Wang Jia-Qi, Wu Li, Zhang Feng, Chen Zhi-Peng, Li Wei-Dong, Cai Hao, Liu Xiao
机构信息
a Department of Pharmacology, College of Pharmacy , Nanjing University of Chinese Medicine , Nanjing 210023 , China.
b National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine , Nanjing 210023 , China.
出版信息
J Asian Nat Prod Res. 2019 Feb;21(2):186-195. doi: 10.1080/10286020.2017.1408594. Epub 2017 Nov 28.
The study was intended to investigate the protective effects of emodin against cell injury and inflammation in AR42J cells. We determined trypsin and lipase activity, intracellular ROS and MMP using specific assay kits. The related protein expression and TNF-α and IL-6 in the medium were assayed by Western blot and ELISA kits. Results showed that emodin could protect AR42J cells against cell injury caused by cerulein and lipopolysaccharide which were possibly associated with inhibition of mitochondrial damage, ROS production, and then significantly inhibited ROS-mediated pathway, and ameliorated pancreatic cells injury by depleting the levels of inflammatory cytokines.
本研究旨在探讨大黄素对AR42J细胞损伤和炎症的保护作用。我们使用特定的检测试剂盒测定了胰蛋白酶和脂肪酶活性、细胞内活性氧（ROS）和线粒体膜电位（MMP）。通过蛋白质免疫印迹法和酶联免疫吸附测定试剂盒检测了相关蛋白表达以及培养基中肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的水平。结果表明，大黄素可保护AR42J细胞免受雨蛙肽和脂多糖引起的细胞损伤，这可能与抑制线粒体损伤、ROS生成有关，进而显著抑制ROS介导的信号通路，并通过降低炎症细胞因子水平改善胰腺细胞损伤。
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