Careaga-Houck M, Sprecher H
Department of Physiological Chemistry, College of Medicine, Ohio State University, Columbus 43210.
J Lipid Res. 1989 Jan;30(1):77-87.
When rats were fed a corn oil versus a corn oil-fish oil diet the overall phospholipid content and composition as well as the subclass distribution of the choline- and ethanolamine-containing glycerophospholipids from neutrophils were not altered. The serine-containing glycerophospholipids were characterized by high levels of stearic and oleic acids. When fish oil was added to the diet it replaced some of the arachidonate in both the inositol- and the serine-containing glycerophospholipids. In the corn oil-fed animals, 25.2 and 33.6 mole %, respectively, of the molecular species of 1,2-diacyl- and 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine contained arachidonate. The values for 1,2-diacyl and 1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine were, respectively, 41 and 55.8 mole %. When half of the 5% corn oil in the diet was replaced by fish oil, there was a 53, 38, 27, and 25% reduction, respectively, in the level of arachidonate in these four lipid subclasses. The amount of 5,8,11,14,17-eicosapentaenoic acid incorporated into these four subclasses was always less than the decline in arachidonic acid. This was due, in part, to the acylation of small amounts of 22-carbon (n-3) acids into these lipids. Molecular species analysis demonstrated that 5,8,11,14,17-eicosapentaenoic acid paired with the same components at the sn-1 position, and in the same ratio, as did arachidonic acid. The amounts of 16- and 18-carbon saturated and unsaturated fatty acid at the sn-2 position were not altered by dietary change. Collectively, these findings suggest that 5,8,11,14,17-eicosapentaenoic and arachidonic acids are metabolized in a similar way by neutrophils. These studies also support the concept that neutrophils contain two metabolic pools of phospholipids. One pool is altered by dietary fat change while the pool containing 16- and 18-carbon acids is resistant to change when fish oil is included in the diet.
当给大鼠喂食玉米油与玉米油 - 鱼油饮食时，中性粒细胞中总磷脂含量、组成以及含胆碱和乙醇胺的甘油磷脂的亚类分布均未改变。含丝氨酸的甘油磷脂的特征在于硬脂酸和油酸含量较高。当在饮食中添加鱼油时，它取代了含肌醇和含丝氨酸的甘油磷脂中的一些花生四烯酸。在喂食玉米油的动物中，1,2 - 二酰基 - 和1 - O - 烷基 - 2 - 酰基 - sn - 甘油 - 3 - 磷酸胆碱的分子种类中分别有25.2%和33.6%的分子含有花生四烯酸。1,2 - 二酰基和1 - O - 烯基 - 1'- 烯基 - 2 - 酰基 - sn - 甘油 - 3 - 磷酸乙醇胺的相应值分别为41%和55.8%。当饮食中5%的玉米油有一半被鱼油取代时，这四个脂质亚类中花生四烯酸的水平分别降低了53%、38%、27%和25%。掺入这四个亚类中的5,8,11,14,17 - 二十碳五烯酸的量总是小于花生四烯酸的减少量。这部分是由于少量22碳（n - 3）酸酰化到这些脂质中。分子种类分析表明，5,8,11,14,17 - 二十碳五烯酸在sn - 1位置与相同的成分配对，且比例与花生四烯酸相同。sn - 2位置的16碳和18碳饱和及不饱和脂肪酸的量不受饮食变化的影响。总体而言，这些发现表明5,8,11,14,17 - 二十碳五烯酸和花生四烯酸在中性粒细胞中的代谢方式相似。这些研究还支持中性粒细胞含有两个磷脂代谢池的概念。一个池会因饮食脂肪变化而改变，而含有16碳和18碳脂肪酸的池在饮食中包含鱼油时对变化具有抗性。
