Ahmadi-Eslamloo Hossein, Moosavi Seyed Mostafa Shid, Dehghani Gholam Abbas
Department of Physiology, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Med Sci. 2017 Nov;42(6):544-552.
Ischemic stroke recovery is poor in diabetic mellitus (DM). Vanadium compounds (vanadium) relieve DM signs, but their influences on cerebral ischemia/reperfusion injury (I/RI) are inconclusive. Herein, the intensity of I/RI was inspected in vanadium-treated DM rats.
Rats made diabetic with a single intravenous dose of streptozocin (39 mg/kg). Normal and DM rats used water or vanadyl solution for 45 days. Under isoflurane anesthesia, right middle cerebral artery occlusion was performed for 60 minutes and 12 hours reperfusion. Ischemic rats were divided into untreated-control normal (ICN) and diabetic (ICD), vanadium-treated normal (IVTN) and diabetic (IVTD) groups (n=14 each). After neurological deficit score (NDS) test, the rats were sacrificed and their brain removed and stained with triphenyltetrazolium chloride (TTC) to measure cerebral infarct volume (CIV, mm) or Evans blue extravasation (EBE, μg/g wet-tissue). Data analysis was performed using one-way ANOVA and Tukey's test (SPSS software, version 21.0) and P values <0.05 were considered statistically significant.
Blood glucose (BG, mg/dL) was similar in ICN and IVTN, elevated in IVTD and ICD (245±6 vs. 344±2, P<0.001). The increased CIV in ICN and IVTN was similar (48±2 and 34±5), very high in ICD but lower in IVTD (249±37 vs. 110±16, P<0.001). EBE was absent in non-lesioned hemispheres, similarly increased in lesioned hemispheres of ICN and IVTN (14±1 and 13±1). EBE in IVTD was significantly lower than ICD (21±2 vs. 33±5, P=0.01).
I/RI was moderate in normoglycemia and did not change with vanadium. Hyperglycemia robustly intensified I/RI. Vanadium ameliorated hyperglycemia and reduced I/RI. Nonetheless, more investigations are required to link the mechanisms of vanadium on DM and stroke injuries.
糖尿病（DM）患者缺血性中风恢复情况较差。钒化合物（钒）可缓解糖尿病症状，但其对脑缺血/再灌注损伤（I/RI）的影响尚无定论。在此，我们检测了钒处理的糖尿病大鼠的I/RI强度。
通过单次静脉注射链脲佐菌素（39mg/kg）使大鼠患糖尿病。正常大鼠和糖尿病大鼠分别饮用蒸馏水或钒溶液45天。在异氟烷麻醉下，对右侧大脑中动脉进行60分钟闭塞并再灌注12小时。将缺血大鼠分为未处理的正常对照组（ICN）和糖尿病对照组（ICD）、钒处理的正常组（IVTN）和糖尿病组（IVTD）（每组n = 14）。在进行神经功能缺损评分（NDS）测试后，处死大鼠，取出大脑并用氯化三苯基四氮唑（TTC）染色以测量脑梗死体积（CIV，mm）或伊文思蓝外渗量（EBE，μg/g湿组织）。使用单因素方差分析和Tukey检验（SPSS软件，版本21.0）进行数据分析，P值<0.05被认为具有统计学意义。
ICN组和IVTN组的血糖（BG，mg/dL）相似，IVTD组和ICD组血糖升高（245±6 vs. 344±2，P<0.001）。ICN组和IVTN组增加的CIV相似（48±2和34±5），ICD组非常高而IVTD组较低（249±37 vs. 110±16，P<0.001）。未损伤半球未出现EBE，ICN组和IVTN组损伤半球的EBE增加相似（14±1和13±1）。IVTD组的EBE显著低于ICD组（21±2 vs. 33±5，P = 0.01）。
正常血糖时I/RI为中度，且不受钒影响。高血糖显著加剧I/RI。钒可改善高血糖并减轻I/RI。尽管如此，仍需要更多研究来阐明钒对糖尿病和中风损伤的作用机制。
