大鼠和人肠道固有层细胞对旋毛虫新生幼虫活力和肌幼虫形成的影响。

Effects of rat and human intestinal lamina propria cells on viability and muscle establishment of Trichinella spiralis newborn larvae.

作者信息

Lee T D, Befus D

机构信息

Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Alberta, Canada.

出版信息

J Parasitol. 1989 Feb;75(1):124-8.

PMID:2918432

Abstract

Although eosinophils and other inflammatory cells from the circulation and peritoneal cavity can damage Trichinella spiralis newborn larvae (NBL) in vitro, the cytotoxic potential of cells from the intestinal lamina propria, a site that may be the first line of defense against NBL migration, is unknown. Accordingly, we examined the interaction between NBL and isolated intestinal lamina propria cells (ILPC), including an enriched eosinophil population, from rats and humans. Rat ILPC killed NBL in vitro only after a prolonged incubation of 6 days. However they strongly adhered to NBL after only 4 hr incubation and prevented muscle establishment of NBL injected intravenously. Human ILPC showed similar adherence as rat ILPC but no killing was seen at the incubation time tested (36 hr).

摘要

尽管来自循环系统和腹腔的嗜酸性粒细胞及其他炎症细胞在体外可损伤旋毛虫新生幼虫（NBL），但肠固有层细胞（可能是抵御NBL迁移的第一道防线）的细胞毒性潜力尚不清楚。因此，我们研究了NBL与从大鼠和人类分离的肠固有层细胞（ILPC，包括富集的嗜酸性粒细胞群体）之间的相互作用。大鼠ILPC仅在延长培养6天后才在体外杀死NBL。然而，它们在孵育仅4小时后就强烈黏附于NBL，并阻止静脉注射的NBL在肌肉中定植。人ILPC表现出与大鼠ILPC相似的黏附性，但在所测试的孵育时间（36小时）未观察到杀伤作用。