肠道嗜酸性粒细胞对旋毛虫新生幼虫的蠕虫毒性反应。
Helminthotoxic responses of intestinal eosinophils to Trichinella spiralis newborn larvae.
作者信息
Lee T D
机构信息
Department of Microbiology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
出版信息
Infect Immun. 1991 Dec;59(12):4405-11. doi: 10.1128/iai.59.12.4405-4411.1991.
Abstract
Because the gastrointestinal lamina propria is the first line of defense against invasion with Trichinella spiralis newborn larvae, we investigated the helminthotoxic characteristics of isolated lamina propria eosinophils. Eosinophils were isolated from the intestinal lamina propria of rats and purified to nearly 90% purity by a combination of velocity sedimentation through Percoll and unit gravity sedimentation through a continuous gradient of bovine serum albumin. Isolated eosinophils were of high viability and responded to surface receptor stimulation. Freshly isolated intestinal eosinophils lacked cytotoxic capacity when incubated with newborn larvae in the presence of specific antiserum. Peritoneal eosinophils from the same rats exhibited 100% helminthotoxicity after 24 h. Cytotoxicity could be stimulated in the intestinal eosinophils by the addition of recombinant murine interleukin-5.
摘要
由于胃肠道固有层是抵御旋毛虫新生幼虫入侵的第一道防线，我们研究了分离出的固有层嗜酸性粒细胞的杀蠕虫毒性特征。从大鼠肠道固有层分离出嗜酸性粒细胞，并通过Percoll速度沉降和牛血清白蛋白连续梯度单位重力沉降相结合的方法将其纯化至纯度近90%。分离出的嗜酸性粒细胞具有高活力，并对表面受体刺激有反应。在特异性抗血清存在的情况下，新鲜分离的肠道嗜酸性粒细胞与新生幼虫孵育时缺乏细胞毒性能力。来自相同大鼠的腹腔嗜酸性粒细胞在24小时后表现出100%的杀蠕虫毒性。通过添加重组小鼠白细胞介素-5可刺激肠道嗜酸性粒细胞产生细胞毒性。
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本文引用的文献
1
Host defense mechanisms against nematode parasites: destruction of newborn Trichinella spiralis larvae by human antibodies and granulocytes.
J Infect Dis. 1981 May;143(5):712-8. doi: 10.1093/infdis/143.5.712.
2
Localization of lysosomal and peroxisomal enzymes in the specific granules of rat intestinal eosinophil leukocytes revealed by immunoelectron microscopic techniques.免疫电子显微镜技术揭示大鼠肠道嗜酸性粒细胞特异性颗粒中溶酶体酶和过氧化物酶体酶的定位。
J Histochem Cytochem. 1984 Mar;32(3):267-74. doi: 10.1177/32.3.6693757.
3
Mucosal mast cells. I. Isolation and functional characteristics of rat intestinal mast cells.黏膜肥大细胞。I. 大鼠肠道肥大细胞的分离及功能特性
J Immunol. 1982 Jun;128(6):2475-80.
4
Activation of complement, the induction of antibodies to the surface of nematodes and the effect of these factors and cells on worm survival in vitro.
Eur J Immunol. 1980 Aug;10(8):594-601. doi: 10.1002/eji.1830100805.
5
Trichinella spiralis: newborn larval migration route in rats reexamined.旋毛虫：对大鼠新生幼虫迁移途径的重新研究
Exp Parasitol. 1986 Feb;61(1):76-85. doi: 10.1016/0014-4894(86)90137-2.
6
Trichinella spiralis: vascular recirculation and organ retention of newborn larvae in rats.旋毛虫：新生幼虫在大鼠体内的血管再循环和器官滞留
Exp Parasitol. 1986 Dec;62(3):430-41. doi: 10.1016/0014-4894(86)90052-4.
7
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9
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