Saberi Eshaghali, Zahedani Shahram Shahraki, Ebrahimipour Sediqe, Valian Neda
Department of Endodontics, Oral and Dental Diseases Research Center, Dental School, Zahedan University of Medical Sciences, Zahedan, Iran.
Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
J Int Soc Prev Community Dent. 2017 Oct;7(Suppl 2):S82-S87. doi: 10.4103/jispcd.JISPCD_205_17. Epub 2017 Oct 30.
Root canal preparation and obturation are of great importance in endodontics. Its purpose is to eliminate pulpal and periradicular disease. The aim of this study was to compare coronal bacterial microleakage in prepared root canals using Neoniti A1 and Reciproc files that obturated with lateral compaction, single cone gutta-percha, and hybrid (tapered cone/lateral compaction) methods.
In this study, a total of 110 single-rooted mandibular first premolars were choose and randomly divided into two study groups A and B (each 45) that one group was prepared with Reciproc and another with Neoniti A1 and negative and positive control groups (each 10). Each group divided into three subgroups of 15 each and obturated using a single cone, lateral compaction, and hybrid (tapered cone/lateral compaction) techniques. For evaluation of coronal leakage, "two-chamber setup" was used. The solution of enterococcus faecalis culture was injected in the upper chamber and incubated. If the bacteria pass through the canal and obturation materials, the lower chamber becomes turbid. TSB medium in the lower chamber (apex) were investigated every day in terms of occurrence of turbidity, and the duration of occurrence of leakage was recorded. The data were analyzed using Chi-square test.
Data analysis showed that in each group the difference in percentages between subgroups was statistically significant ( = 0.003). So that the highest and the lowest amount of leakage in both groups were related to lateral compaction and hybrid techniques, respectively.
Under the conditions of this study, independent of the instrument used for canal preparation, hybrid method and then single-cone technique, however, were more effective in the prevention of coronal leakage than lateral condensation technique.
根管预备和充填在牙髓病学中至关重要。其目的是消除牙髓和根尖周疾病。本研究的目的是比较使用Neoniti A1锉和Reciproc锉预备根管后，采用侧向加压、单尖牙胶尖和混合（锥度锥/侧向加压）方法充填时的冠部细菌微渗漏情况。
在本研究中，共选取110颗单根下颌第一前磨牙，随机分为两个研究组A和B（每组45颗），一组用Reciproc锉预备根管，另一组用Neoniti A1锉预备根管，以及阴性和阳性对照组（每组10颗）。每组再分为三个亚组，每组15颗，分别采用单尖、侧向加压和混合（锥度锥/侧向加压）技术进行充填。为评估冠部渗漏情况，采用“双腔设置”。将粪肠球菌培养液注入上腔并孵育。如果细菌穿过根管和充填材料，下腔会变浑浊。每天观察下腔（根尖）中的TSB培养基是否出现浑浊，并记录渗漏发生的持续时间。数据采用卡方检验进行分析。
数据分析表明，每组亚组之间的百分比差异具有统计学意义（P = 0.003）。因此，两组中渗漏量最高和最低的分别与侧向加压和混合技术有关。
在本研究条件下，无论用于根管预备的器械如何，混合方法其次是单尖技术，在预防冠部渗漏方面比侧向加压技术更有效。
