Yadav Ankit Kumar, Sadora Manik, Singh Sachin Kumar, Gulati Monica, Maharshi Peddi, Sharma Abhinav, Kumar Bimlesh, Rathee Harish, Ghai Deepak, Malik Adil Hussain, Garg Varun, Gowthamrajan K
Department of Pharmaceutics, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India.
Department of Pharmaceutics, J.S.S. College of Pharmacy, Ooty, Tamil Nadu, India.
J Adv Pharm Technol Res. 2017 Oct-Dec;8(4):150-155. doi: 10.4103/japtr.JAPTR_70_17.
To overcome the limitations of the conventionally used methods for evaluation of orally administered colon-targeted delivery systems, a novel dissolution method using probiotics has been recently reported. In the present study, universal suitability of this medium composed of five different probiotics is established. Different delivery systems - mini tablets, liquisolid compacts, and microspheres coated with different polysaccharides - were prepared and subjected to sequential dissolution testing in medium with and without microbiota. The results obtained from fluid thioglycollate medium (FTM)-based probiotic medium for all the polysaccharide-based formulations showed statistically similar dissolution profile to that in the rat and goat cecal content media. Hence, it can be concluded that the developed FTM-based probiotic medium, once established, may eliminate the need for further animal sacrifice in the dissolution testing of polysaccharide-based colon-targeted delivery system.
为克服传统用于评估口服结肠靶向给药系统的方法的局限性，最近报道了一种使用益生菌的新型溶出方法。在本研究中，确定了这种由五种不同益生菌组成的培养基的普遍适用性。制备了不同的给药系统——迷你片、液固型制剂和涂覆不同多糖的微球，并在有和没有微生物群的培养基中进行连续溶出测试。从基于巯基乙酸盐流体培养基（FTM）的益生菌培养基中获得的所有基于多糖的制剂的结果显示，其溶出曲线与大鼠和山羊盲肠内容物培养基中的溶出曲线在统计学上相似。因此，可以得出结论，所开发的基于FTM的益生菌培养基一旦确定，可能无需在基于多糖的结肠靶向给药系统的溶出测试中进一步进行动物处死。
