Ewald Maria Paula de Carvalho, Martins Felippe Danyel Cardoso, Caldart Eloiza Teles, Vieira Fernando Emmanuel Gonçalves, Yamamura Milton Hissashi, Sasse João Pedro, Barros Luiz Daniel de, Freire Roberta Lemos, Navarro Italmar Teodorico, Garcia João Luis
Laboratório de Protozoologia, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
Laboratório de Parasitologia, Centro de Ciências Humanas e da Educação, Universidade Estadual do Norte do Paraná - UENP, Jacarezinho, PR, Brasil.
Rev Bras Parasitol Vet. 2017 Oct-Dec;26(4):472-478. doi: 10.1590/S1984-29612017068. Epub 2017 Nov 27.
Rearing free-range chicken is based on grazing feeding patterns, and these animals could be potential environmental contaminants of Cryptosporidium oocysts for humans and other animals. Therefore, the present study aimed to evaluate the molecular prevalence of Cryptosporidium spp. in free-range chickens from Brazil. A total of 351 fecal samples from chickens were examined from 20 farms. For detection of Cryptosporidium spp., 18S rRNA gene fragments were amplified using a nested PCR reaction. Positive samples were sent for sequencing. The overall prevalence of Cryptosporidium was 25.6% (95% CI = 21.2% - 30.6%). Sequencing of the amplified fragments allowed for the identification of three species: C. meleagridis in 57 (62.6%), C. baileyi in 15 (16.4%), C. parvum in 3 (3.2%) samples, and a new Cryptosporidium genotype (C. genotype BrPR1) in 3 (3.2%) samples. Cryptosporidium genotype BrPR1 has not yet been classified as a species, and its host spectrum is not known. Cryptosporidium, including zoonotic species, exists at a high prevalence in free-range chickens within the region studied.
饲养散养鸡是基于放牧饲养模式，这些动物可能成为隐孢子虫卵囊对人类和其他动物的潜在环境污染物。因此，本研究旨在评估巴西散养鸡中隐孢子虫属的分子流行情况。从20个农场共采集了351份鸡的粪便样本。为检测隐孢子虫属，使用巢式PCR反应扩增18S rRNA基因片段。阳性样本送去测序。隐孢子虫的总体流行率为25.6%（95%置信区间=21.2%-30.6%）。扩增片段的测序鉴定出三个物种：57份样本（62.6%）为火鸡隐孢子虫，15份样本（16.4%）为贝利隐孢子虫，3份样本（3.2%）为微小隐孢子虫，3份样本（3.2%）为一种新的隐孢子虫基因型（隐孢子虫基因型BrPR1）。隐孢子虫基因型BrPR1尚未被归类为一个物种，其宿主谱也未知。在所研究地区的散养鸡中，包括人畜共患物种在内的隐孢子虫普遍存在。
