中国和田黑鸡中隐孢子虫 spp. 的遗传特征显示出两种新型的隐孢子虫 meleagridis 亚型。
Genetic characterization of Cryptosporidium spp. in Hotan Black Chickens in China reveals two novel subtypes of Cryptosporidium meleagridis.
机构信息
College of Animal Science and Technology, Tarim University, Alar, Xinjiang 843300, China.
Xinjiang Agricultural Vocational Technical College, Changji, Xinjiang 831199, China.
出版信息
Parasite. 2022;29:50. doi: 10.1051/parasite/2022051. Epub 2022 Nov 9.
A total of 617 fecal specimens were collected on 18 Hotan Black chicken farms in Southern Xinjiang, China, and tested for the presence of Cryptosporidium spp. by PCR of the small subunit ribosomal RNA (SSU rRNA) gene. The overall infection rate by Cryptosporidium spp. was 11.5% (71/617), and ten of the 18 farms were positive. The infection rate by Cryptosporidium spp. was 14.5% (48/331) in the 30-60 d group, higher than chickens in the <30 d (12.0%, 15/125), 60-90 d (6.9%, 5/72), and >90 d (3.4%, 3/89) groups. Cryptosporidium meleagridis (n = 38) and C. baileyi (n = 33) were confirmed by sequencing analysis. A total of 25 of the 38 C. meleagridis-positive specimens were subtyped successfully at the gp60 gene, including one known subtype (IIIbA23G1R1, n = 1) and two novel subtypes, named IIIbA25G1R1 (n = 20) and IIIbA31G1R1 (n = 4). The results showed that infection by Cryptosporidium spp. in Hotan Black Chickens was common in this area and the distribution of C. meleagridis subtypes had regional characteristics.
在中国新疆南部的 18 个和田黑鸡养殖场采集了 617 份粪便标本，通过聚合酶链反应（PCR）检测小亚单位核糖体 RNA（SSU rRNA）基因检测隐孢子虫属的存在。隐孢子虫属的总感染率为 11.5%（71/617），其中 18 个养殖场中有 10 个呈阳性。30-60 日龄组的隐孢子虫属感染率为 14.5%（48/331），高于<30 日龄（12.0%，15/125）、60-90 日龄（6.9%，5/72）和>90 日龄（3.4%，3/89）组。通过测序分析，确认了隐孢子虫属（n=38）和 C. baileyi（n=33）。在 38 份 C. meleagridis 阳性标本中，有 25 份成功进行了 gp60 基因的亚分型，包括一个已知亚型（IIIbA23G1R1，n=1）和两个新亚型，命名为 IIIbA25G1R1（n=20）和 IIIbA31G1R1（n=4）。结果表明，和田黑鸡在该地区普遍感染隐孢子虫属，C. meleagridis 亚型的分布具有区域性特征。
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