一种利用介孔氧化镁作为微载体提高天然羟基磷灰石抗肝癌细胞系 HepG2 抗癌活性的新方法。

A Novel Method to Improve the Anticancer Activity of Natural-Based Hydroxyapatite against the Liver Cancer Cell Line HepG2 Using Mesoporous Magnesia as a Micro-Carrier.

机构信息

Department of Chemistry, Science College, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia;

Department of Biology, Science College, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia

出版信息

Molecules. 2017 Nov 24;22(12):1947. doi: 10.3390/molecules22121947.

Micro-carriers are the best known vehicles to transport different kinds of drugs to achieve high impact. In this study, mesoporous magnesium oxide has been harnessed as a micro-carrier to encapsulate the anticancer candidate drug natural-based cubic hydroxyapatite (HAP). HAP@MgO composites with different HAP loading (0-60 wt %), were prepared by a hydrothermal treatment method using triethanol amine as a template. The characterization of the prepared composites were achieved by using XRD, Raman spectroscopy, FTIR and SEM. Characterization data confirm the formation of sphere-like structures of MgO containing HAP particles. It was observed that the size of the spheres increased with HAP loading up to 40 wt %, then collapsed. Furthermore, the anticancer property of the prepared composites was evaluated against the HepG2 liver cancer cell line. The HAP@MgO composites exhibited higher activity than neat MgO or HAP. The 20 wt % of HAP was the optimum loading to control cell proliferation by inducing apoptosis. Apoptosis was determined by typical apoptotic bodies produced by the cell membrane.

微载体是将各种药物输送到体内以达到高疗效的最常见载体。在这项研究中，介孔氧化镁被用作微载体来包封抗癌候选药物天然立方羟基磷灰石（HAP）。通过水热处理法，使用三乙醇胺作为模板，制备了不同 HAP 负载量（0-60wt%）的 HAP@MgO 复合材料。通过 XRD、拉曼光谱、FTIR 和 SEM 对制备的复合材料进行了表征。表征数据证实了含有 HAP 颗粒的 MgO 球型结构的形成。观察到，随着 HAP 负载量增加到 40wt%，球体的尺寸增加，然后坍塌。此外，还评估了制备的复合材料对 HepG2 肝癌细胞系的抗癌性能。与纯 MgO 或 HAP 相比，HAP@MgO 复合材料表现出更高的活性。20wt%的 HAP 是通过诱导细胞凋亡来控制细胞增殖的最佳负载量。通过细胞膜产生的典型凋亡小体来确定细胞凋亡。