Sakaguchi M, Inouye S, Miyazawa H, Kamimura H, Kimura M, Yamazaki S
Department of Microbiology, Institute of Public Health, Tokyo, Japan.
Lab Anim Sci. 1989 Jan;39(1):63-6.
The efficiency of various dust respirators for eliminating mouse allergens [mouse urine proteins (MUP), pelts proteins (MPP) and serum albumin (MSA)] were evaluated with use of low-volume air samplers and immunochemical methods. Three kinds of dust respirators from one manufacturer which have different efficacy in the exclusion of dust particles were put on the fiber glass filter in each air sampler. Then the air in a mouse housing room was sampled. The allergens passed through the respirators, were trapped in the fiber glass filters, and then extracted from the filters. The allergens of MUP and MPP in the extract were measured by an inhibition method of fluorometric enzyme-linked immunosorbent assay (ELISA) for IgE antibody and those of MSA measured by a fluorometric sandwich ELISA. The respirator with the lowest capability of exclusion was found to eliminate 65-86% of respective allergens. The other two respirators with higher powers eliminated 98% of MUP. MPP and MSA were eliminated to undetectable levels through these respirators. This study provided a means for the evaluation of dust respirators for animal aeroallergens.
使用低流量空气采样器和免疫化学方法评估了各种防尘呼吸器清除小鼠过敏原[小鼠尿蛋白（MUP）、皮毛蛋白（MPP）和血清白蛋白（MSA）]的效率。将来自同一制造商的三种对尘粒排除效果不同的防尘呼吸器放置在每个空气采样器的玻璃纤维滤器上。然后对小鼠饲养室内的空气进行采样。过敏原穿过呼吸器，被困在玻璃纤维滤器中，然后从滤器中提取出来。提取物中MUP和MPP的过敏原通过荧光酶联免疫吸附测定（ELISA）IgE抗体的抑制法进行测量，而MSA的过敏原通过荧光夹心ELISA进行测量。发现排除能力最低的呼吸器能清除65 - 86%的相应过敏原。另外两种能力更强的呼吸器能清除98%的MUP。MPP和MSA通过这些呼吸器被清除到检测不到的水平。本研究提供了一种评估动物气传过敏原防尘呼吸器的方法。
