Zhu L, Huang L, Zhu Y, Ding X, Zhu G
Acta Virol. 2017;61(4):487-491. doi: 10.4149/av_2017_412.
Calcium is one of the most prominent second messengers in eukaryotic cells. The involvement of calcium signaling in bovine herpesvirus 1 (BoHV-1) replication was not yet reported. In this study, we revealed that the L-type Ca2+ calcium channel blocker, Verapamil and store-operated calcium channel blocker, 2-aminoethyl diphenylborinate (2-APB) inhibited BoHV-1 replication in MDBK cells at the post-entry stages, and the Na+/Ca²+ exchanger inhibitor, N-arachidonoyl glycine exchanger (NAGly) interfered with the viral entry process. NAGly also effected the phosphorylation of PLCγ-1 at Ser1248, which corroborated our previous findings, that PLCγ-1 is important for BoHV-1 entry. Collectively, these results suggest that diverse calcium channels are employed by BoHV-1 for efficient replication.
钙是真核细胞中最显著的第二信使之一。钙信号传导参与牛疱疹病毒1型（BoHV-1）复制的情况尚未见报道。在本研究中，我们发现L型Ca2+钙通道阻滞剂维拉帕米和储存操纵性钙通道阻滞剂2-氨基乙基二苯基硼酸盐（2-APB）在进入后阶段抑制MDBK细胞中的BoHV-1复制，而Na+/Ca²+交换体抑制剂N-花生四烯酰甘氨酸交换体（NAGly）干扰病毒进入过程。NAGly还影响PLCγ-1在Ser1248处的磷酸化，这证实了我们之前的发现，即PLCγ-1对BoHV-1进入很重要。总体而言，这些结果表明BoHV-1利用多种钙通道进行高效复制。
