Vecino Elena, Urcola Haritz, Bayon Alejando, Sharma Sansar C
Experimental Ophathalmo-Biology Group, Department Cell Biology and Histology, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Vizcaya, Spain.
Ophthalmology Section, Hospital Universitario Araba, Vitoria, Spain.
Methods Mol Biol. 2018;1695:41-48. doi: 10.1007/978-1-4939-7407-8_4.
Two methods to induce elevation of the intraocular pressure (experimental glaucoma) are described in the present chapter. The first method is based on increasing the post-trabecular resistance to aqueous outflow by cauterizing the episcleral veins (EVC). This method allows the observation of ultrastructural changes in the trabecular meshwork (TM) without interfering with any structure within the eye such as TM, ciliary body, and/or the Retina. The second method is the multiple injection of microbeads into the anterior chamber, as a pre and intra-trabecular method that induce secondary effects on the TM cells. Both methods lead to an increase in IOP.
本章介绍了两种诱导眼压升高（实验性青光眼）的方法。第一种方法是通过烧灼巩膜静脉（EVC）增加小梁后段对房水流出的阻力。这种方法可以观察小梁网（TM）的超微结构变化，而不会干扰眼内的任何结构，如TM、睫状体和/或视网膜。第二种方法是向前房多次注射微珠，作为一种小梁前和小梁内方法，对TM细胞产生继发性影响。两种方法都会导致眼压升高。
