Calkins David J, Lambert Wendi S, Formichella Cathryn R, McLaughlin William M, Sappington Rebecca M
The Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
Department of Ophthalmology and Visual Sciences, Vanderbilt University School of Medicine, Nashville, TN, USA.
Methods Mol Biol. 2018;1695:23-39. doi: 10.1007/978-1-4939-7407-8_3.
Glaucoma is a common optic neuropathy that leads to vision loss through the degeneration of retinal ganglion cells (RGCs) and their axons. RGC degeneration in glaucoma is associated with sensitivity to intraocular pressure (IOP) and elevated IOP (also known as ocular hypertension) is the primary modifiable risk factor. Ocular hypertension is the primary characteristic of rodent models for glaucoma research. Intracameral injection of microbeads has evolved as a preferred method of IOP elevation in rodents, particularly in mice. Here, we outline the protocol and method for the Microbead Occlusion Model in mice. We highlight the importance of anesthesia choice and the utilization of glass micropipettes in combination with a micromanipulator and microsyringe pump for the successful execution of the model.
青光眼是一种常见的视神经病变，可通过视网膜神经节细胞（RGCs）及其轴突的退化导致视力丧失。青光眼中的RGC退化与眼内压（IOP）敏感性相关，而眼压升高（也称为高眼压症）是主要的可改变风险因素。高眼压症是青光眼研究啮齿动物模型的主要特征。前房内注射微珠已发展成为啮齿动物，特别是小鼠眼压升高的首选方法。在这里，我们概述了小鼠微珠阻塞模型的方案和方法。我们强调了麻醉选择的重要性，以及结合使用玻璃微量移液器、显微操作器和微量注射泵以成功实施该模型的重要性。
